Mon1 (Drosophila melanogaster, Q9VR38) notes

The falcon deep research report (Mon1-deep-research-falcon.md) arrived after the wrapper timeout; it additionally summarises fly fat-body autophagy data (Mon1 null cells fail to recruit Rab7 to autophagosomes).

Identity

• Dmon1/Sand1 family; catalytic-core subunit (with Ccz1) of the Mon1-Ccz1-Bulli (RMC1) Rab7 GEF that drives Rab5-to-Rab7 conversion on maturing endosomes.

Literature

• PMID:23418349; PMID:23418349
• Trimeric GEF PMID:32499409; PMID:32499409
• Cryo-EM of Drosophila Mon1-Ccz1-RMC1 PMID:37216550
• Regulation: PMID:37463208; PMID:37463208
• Synapse: PMID:26290519

Curation thoughts

• Protein binding (Rab5) -> MODIFY to small GTPase binding (Rab5 recruits/activates the GEF on membranes).
• GEF inhibitor activity reflects intramolecular autoinhibition by the Mon1 N-terminus of its own complex -> over-annotation as an MF.
• Synaptic cleft (IDA) cannot be verified from the abstract; a cytosolic GEF subunit in the cleft is unexpected -> UNDECIDED.