TreeGrafter-generated GO annotations
UniProt record for yorkie (F2UDK1)
-
PANTHER places F2UDK1 in a MAGI-related subfamily, not the YAP1 family.
"PANTHER; PTHR10316:SF68; AGAP003128-PA; 1."
F2UDK1 motif and identity checks (reviewer analysis)
-
F2UDK1 has four HXRXXS motifs.
"F2UDK1 (714 aa) carries four HXRXXS motifs"
-
No recognizable TEAD-binding motif was found in F2UDK1.
"We find **no recognizable TEAD-binding motif** in F2UDK1."
-
F2UDK1 resembles YAP1, Yorkie and coYki more than F2U5K0 does.
"F2UDK1 is the better match to all three Yorkie/YAP proteins."
A fast and robust gene knockout method for <i>Salpingoeca rosetta</i> informs the genetics of choanoflagellate multicellular development
-
yorkie knockout cells proliferate at a wild-type rate.
"Growth curves indicated that yorkiepac1 KO cells proliferated at a similar rate to the wild type"
-
yorkie knockout rosettes are of normal size.
"The size of hippopac1 and yorkiepac1 rosettes did not significantly differ from wild type (11.6 ± 1.7 cells and 9.8 ± 3.1 cells"
-
All knockout clones still develop into rosettes.
"all six KO clones reliably developed into rosettes, as did wild-type cells"
A selection-based knockout approach for a choanoflagellate reveals regulation of multicellular development by Hippo signaling.
-
Warts and Yorkie regulate extracellular matrix genes, including couscous.
"RNA sequencing revealed that Warts and Yorkie regulated several extracellular matrix genes involved in multicellularity (including couscous)"
Assembling a Hippo: the evolutionary emergence of an animal developmental signaling pathway.
-
S. rosetta Yorkie has two WW domains and four putative Warts sites.
"like most animals, srYki has 2 WW domains, whereas mbYki has 1, and srYki has 4 putative Warts phosphorylation sites"
-
The srYki TEAD-binding domain lacks conserved alpha2-helix residues.
"the srYki TBD shows less conservation with Yorkie/YAP/TAZ than coYki, with srYki lacking conserved residues within the α2 helix that are critical for YAP-TEAD interaction in mammals"
-
Whether choanoflagellate Yorkie homologs bind Sd/TEAD is unclear.
"The degree to which choanoflagellate Yorkie orthologs interact with Sd/TEAD proteins is therefore currently unclear"
Premetazoan origin of the hippo signaling pathway.
-
S. rosetta has a Yorkie homolog that clusters with metazoan Yorkie.
"We have identified clear Yki homologs in two independent non-metazoan lineages, the filastereans (Capsaspora owczarzaki, from herein called “Capsaspora”) and the choanoflagellates (Monosiga brevicollis and Salpingoeca rosetta) (Figure 1B)."
-
All non-metazoan Yki homologs, including S. rosetta's, keep the N-terminal TEAD-interaction region.
"Importantly, all these non-metazoan Yki homologs contain highly conserved functional sites like the Hippo pathway responsive phosphorylation site S168/127 and the N-terminal homology region that is critical for interaction with Sd/TEAD transcription factor (Figure 2A)."