Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PSEPK gatA
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GatA is the amidase-family subunit of heterotrimeric GatABC.
"SUBUNIT: Heterotrimer of A, B and C subunits."
Direct glutaminyl-tRNA biosynthesis and indirect asparaginyl-tRNA biosynthesis in Pseudomonas aeruginosa PAO1.
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Native PAO1 uses direct GlnRS and indirect Asn formation; its GatABC also transamidates supplied heterologous Glu-tRNA(Gln) in vitro. Do not infer native KT2440 Gln-transamidation flux from that capacity.
"The heterotrimeric AdT of P. aeruginosa has no Glu-tRNA Gln substrate in its host, but it can transamidate heterologous Glu-tRNA Gln ."
Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis.
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PAO1 transamidosome structure and kinetics distinguish stabilization of the mischarged Asp-tRNA substrate from product release. This is a comparative architectural basis, not a native KT2440 complex or error-rate measurement.
"However, Pa GatCAB does protect Asp-tRNA Asn from deacylation"
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
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Native B. subtilis GatCAB activity is present in soluble S100 extracts; this is comparative broad-compartment evidence for conserved GatA machinery, distinct from recombinant E. coli purification.
"The antibodies were able to neutralize the activity of the pure heterotrimeric Glu-AdT and also destroy all enzyme activity in crude B. subtilis S100 extracts (data not shown)."