GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

InterPro2GO supplies family/domain-based automated annotations that require curator review against the specific TtgA adaptor protein context.

GO_REF:0000118

TreeGrafter-generated GO annotations

TreeGrafter transfers GO terms from phylogenetic families; curator review checks whether the transferred term matches the P. putida TtgA ortholog.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

The combined UniProt automated pipeline annotation provides IEA terms that must be checked for specificity and evidence fit.

PMID:12743835

Comparative genomic analysis of solvent extrusion pumps in Pseudomonas strains exhibiting different degrees of solvent tolerance

Solvent tolerance differences across Pseudomonas strains correlate with the number and type of RND solvent extrusion pumps.

"We also studied the number and type of efflux pumps in different strains in comparison with the P. putida DOT-T1E strain."

PMID:14506010

Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR

TtgABC is a broad-substrate RND efflux pump whose expression is induced by hydrophobic antibiotics.

"The TtgABC efflux pump, which has a broad substrate specificity, extrudes antibiotics such as ampicillin, carbenicillin, tetracycline, nalidixic acid, and chloramphenicol."

PMID:32296906

Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations

KT2440 carries only the TtgABC member of the Ttg solvent-efflux systems.

"the frequently used KT2440 strain contains only the TtgABC system"
TtgA is the lipoprotein adaptor subunit of the TtgABC pump.

"a lipoprotein that plays a role in stabilizing the interaction between the other two elements (TtgA/D/G)"
Loss of ttgA lowers efflux activity and compromises tolerance to several monoterpenoid toxicants.

"the deletion in the ttgA gene results in a loss of the TtgABC efflux system accompanied by a reduced resazurin efflux activity"

file:PSEPK/ttgA/ttgA-uniprot.txt

UniProt entry Q88N30

UniProt identifies TtgA as a probable periplasmic linker protein component of the TtgABC efflux pump.
UniProt places TtgA in the membrane fusion protein family and annotates it as a lipid-anchored inner-membrane protein.

file:PSEPK/ttgA/ttgA-deep-research-falcon.md

Falcon deep research report for ttgA in Pseudomonas putida KT2440

ttgA encodes the membrane fusion or periplasmic adaptor subunit of the TtgABC tripartite RND efflux pump in KT2440.
KT2440 genetic evidence supports a role for TtgABC in efflux-mediated resistance to antibiotics and other xenobiotic toxicants.