Deep research attempts: just deep-research PSEPK fliM --provider perplexity failed ("Provider 'perplexity' not available"); just mod-description-deep-research PSEPK fliM skipped (no MOD provider configured for PSEPK). No provider deep-research file exists for this gene; review based on the UniProt record, cached publications and the project synthesis projects/P_PUTIDA/deep-research/PSEPK__bacterial_chemotaxis_signal_transduction__ppu02030-deep-research-openscientist.md.
TrEMBL entry Q88EU5 (PP_4358), FliM family (PIRSF002888, TIGR01397). No KT2440 fliM experiments cached; family evidence from PMID:31452860 (full text review).
- PMID:31452860
- PMID:31452860
- PMID:31452860
- PMID:36409076 (introduction statement citing earlier work).
Decisions: GO:0003774 cytoskeletal motor activity MODIFY -> GO:0005198 structural molecule activity (lateral: torque made by stator/FliG, FliM is structural/switch); plasma membrane KEEP_AS_NON_CORE (peripheral); flagellum and basal body MODIFY -> GO:0009433 C ring; positive chemotaxis MODIFY -> GO:0006935 (switch serves both directions); cell motility ACCEPT; swarming MARK_AS_OVER_ANNOTATED (consistent with PSEPK motA).
Module: complex-level GO:0005198 for C ring; recorded GO:0005198 as FliM's own best-supported MF. Considered GO:0140031 phosphorylation-dependent protein binding for CheY-P docking but did not add (binding term, family-level only).
Reviewed every existing annotation and the biological summary/core functions. Original annotation payloads, including historical NEW proposals, are preserved. Family/domain evidence supports conserved structural assignments; comparative Salmonella or other bacterial assays are not presented as KT2440 experiments. Exact subunit counts and interaction geometries are not transferred as target measurements.
The normal Falcon research attempt failed (HTTP 403; provider output was not fabricated). This entry records manual review of the cached primary/comparative literature.
General flagellar motility does not by itself establish swarming. The electronically transferred swarming term is qualified against the KT2440 physiological context in PMID:36409076, not rejected because an experimental source is unavailable.
GO:0003774 explicitly includes electrochemical-gradient-driven flagellar rotation. Rotor/switch structural roles are distinguished from autonomous torque generation. The C-ring/MS-ring CC terms were verified against ontology ancestry and remain valid substructure locations.