vwc2 (brorin) notes

ZFIN vwc2 (ZDB-GENE-090313-315, chromosome 13; synonym brorin, si:dkey-4j1.1); NCBI Gene 798521;
Ensembl ENSDARG00000076495; UniProt B0I1T8 (309 aa). PANTHER (PTHR46252) pairs it with
si:dkey-283b1.7 as a TGD_tree 1:1 pair, with human ortholog VWC2 (LDO). The separate brorin-like gene
vwc2l (ZDB-GENE-081104-169) is the VWC2L ortholog and is not part of this pair. Reviewed as part of
DANRE_DUPLICATION batch 3 (random sample).

Deep research

Deep research was not available for this batch (Edison returned 402 Payment Required, and the
OpenAI key is invalid). I searched Europe PMC myself for "vwc2 AND zebrafish",
"brorin AND zebrafish" and "si:dkey-283b1.7".

Literature

• Miyake et al. 2017 (PMID:28448525), the only functional study of zebrafish vwc2.
• Identity: PMID:28448525
• Synteny with mouse: PMID:28448525
• Expression: PMID:28448525
• BMP antagonism in vivo: PMID:28448525 and PMID:28448525
• Forebrain phenotypes (two splice MOs, mRNA rescue): PMID:28448525 and PMID:28448525
• vwc2 and vwc2l overlap partly: PMID:28448525
• si:dkey-283b1.7 is not mentioned anywhere in the paper.
• Miwa et al. 2009 (PMID:19852960), abstract only. The paper is about Brorin-like (vwc2l):
  PMID:19852960
  The 2017 paper says the earlier work reported the brorin-like knockdown, and that the role of
  Brorin itself had not been elucidated:
  PMID:28448525
  ZFIN's IMP (nervous system development) on vwc2 from this paper has no morpholino in WITH/FROM,
  while vwc2l carries two morpholinos from the same paper (QuickGO, 2026-09-28). I marked it UNDECIDED
  rather than REMOVE, because the full text is not available.
• Mouse background: Brorin is secreted and inhibits BMP2/6
  PMID:17400546.
  It is a Noelin-recruited extracellular constituent of AMPA receptor complexes:
  PMID:37591201

Analyses (file:DANRE/si_dkey-283b1.7/si_dkey-283b1.7-bioinformatics/RESULTS.md)

• The brorin MOs are vwc2-specific (0 mismatches). They have 8-10 mismatches to si:dkey-283b1.7 and vwc2l.
• The vwc2 core is 83.6% identical to human VWC2 and 89.3% to gar vwc2. The partner si:dkey-283b1.7 is
  only 32.6% identical to vwc2 over the full length.
• Bgee: vwc2 has brain-dominated calls; si:dkey-283b1.7 has calls in retina and brain.

Decisions

• BMP negative regulation (IBA), extracellular (IBA, IEA), forebrain development (IMP): ACCEPT.
• AMPAR complex (IBA) and synapse (IEA): KEEP_AS_NON_CORE.
• Nervous system development (IMP, PMID:19852960): UNDECIDED.