FAU1 (YER183C) notes

UniProt P40099; 5-formyltetrahydrofolate cyclo-ligase / MTHFS, EC 6.3.3.2 [UniProt:P40099].

Evidence journal

• Identified by homology to human MTHFS; recombinant enzyme active PMID:11923304.
• Physiological role: folinic acid utilisation PMID:11923304; fau1 cells accumulate 5-CHO-THF PMID:11923304.
• SHMT is the in vivo source of 5-CHO-THF PMID:11923304.
• Location: mitochondrial proteomics only [PMID:14576278; PMID:16823961; UniProt:P40099]. Cytosol is a YeastPathways default.

Pathway context

YeastPathways PWY-2201 step 5-CHO-THF + ATP -> 5,10-CH=THF (EC 6.3.3.2) is correct.

Decisions

• All MF/BP rows ACCEPT; cytosol RCA KEEP_AS_NON_CORE.