Gene Ontology annotation through association of InterPro records with GO terms
Use of the ND evidence code for Gene Ontology (GO) terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.
Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP.
Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.
The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p.
Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly.
prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast.
High-quality binary protein interaction map of the yeast interactome network.
Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing.
The social and structural architecture of the yeast protein interactome.
Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry.
Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals.
Interactions within the yeast Sm core complex: from proteins to amino acids.
A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins.
Structure-function analysis and genetic interactions of the SmG, SmE, and SmF subunits of the yeast Sm protein ring.
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Yeast SmG Phe35-Asn37-Arg64 forms the Sm-site uridine-binding triad; single Ala mutants are viable, compound mutants are ts, and they are synthetically lethal with RNA-site mutants of other Sm subunits.
"Thus, compound mutations of the SmG RNA binding site elicited a progressive temperature-sensitive (ts) growth defect."
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The Sm ring tolerates loss of one subunit RNA site but not two.
"These all-against-all results for SmG suggest that six intact RNA binding sites in the Sm ring can suffice for in vivo function, but five sites may not."
Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain.
Composition and functional characterization of the yeast spliceosomal penta-snRNP.
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.
UniProtKB/Swiss-Prot entry P40204 (RUXG_YEAST)