"Fbxo41 associated with Skp1 and Cullin1, albeit less efficiently than Fbxo21 which was included as a positive control. Deleting (Fbxo41ΔF-box) or mutating (Fbxo41W577A) the F-box domain abolished these interactions"

"Super-resolution stochastic optical reconstruction microscopy (STORM) revealed that Fbxo41-EGFP resides adjacent to centrosomal protein 135 (Cep135), a centriole proximal-end protein"

"this suggests that both the Coiled-coil and the F-box domains are required for centrosome targeting"

"using two independent model systems and three different cilia markers, we demonstrate that increased Fbxo41 expression robustly disassembles primary cilia"

"these results demonstrate that Fbxo41 requires a functional F-box domain for centriole targeting and cilia disassembly"

"rearrangements of the actin-cytoskeleton are required for Fbxo41 mediated cilia disassembly, and that ciliary disassembly occurs via different mechanisms in mitotic cells and neurons"

"these data indicate that Fbxo41-indcued cilia disassembly affects their signaling capacity in mitotic cells and, albeit to a lesser extent, in neurons"

"we identify FBXO41 as a novel CNS-specific F-box protein that localizes to the centrosome and the cytoplasm of neurons and demonstrate that cytoplasmic FBXO41 promotes neuronal migration"

"We found FBXO41 to be expressed in CNS tissues including cortex, hippocampus, cerebellum, and spinal cord, but absent from the peripheral nervous system tissue such as sciatic nerve and from non-neural tissues"

"we discovered a conspicuous dot-like accumulation of FBXO41 near the nucleus, which we later confirmed to be the centrosome because GFP-FBXO41 colocalized with the centrosomal marker pericentrin 2"

"we refer to it as centrosomal targeting region (CTR, aa 179–354) hereafter"

"we found that significantly more FBXO41 knock-down neurons were stalled in the EGL/ML"

"Collectively, these data indicate that the FBXO41 RNAi-induced phenotype is specific to FBXO41 and suggest that cytoplasmic FBXO41 is crucial to stimulate the migration of cerebellar granule neurons"

"mice displayed an abnormal gait characterized by dragging of the hind limbs, impaired balance, and tremors"

"although the migrating cells eventually catch up and reach the IGL, FBXO41 is required to maintain a normal migration speed in the cerebellum"

"found little or no increase of apoptotic cells in the cortex but a significant increase in the hippocampus and the cerebellum"

"These results support a prosurvival role for FBXO41 in neurons."

"In neuronal/cell models, FBXO41 associates with **SKP1** and **CUL1** (SCF complex components). Removing the F-box (Fbxo41ΔF-box) or introducing an F-box point mutation (W577A) abolishes SKP1/CUL1 interactions, indicating the F-box is required for SCF recruitment."

"Mouse studies report FBXO41 is cytoplasmic and excluded from nucleus, and localizes prominently near the nucleus at the **centrosome** (pericentrin-positive). FBXO41 can be recovered with centrosome-enriched fractions, supporting authentic centrosomal association."

"increased centriolar accumulation of Fbxo41 **promotes primary cilia disassembly/shortening** and affects **Sonic hedgehog (Shh)** signaling, a canonical ciliary pathway. Levels of centrosomal Fbxo41 inversely correlate with cilia length, and disrupting SCF assembly or centrosome targeting disrupts function."

"Loss of Fbxo41 produces an ataxia-like phenotype with neuronal migration defects and cerebellar degeneration. FBXO41 is detected early in embryogenesis (E10) and is abundant postnatally in cerebellum; it is not detected in astrocytes or oligodendrocytes in those studies, supporting neuron-enriched expression."

"a definitive, broadly validated human substrate repertoire for FBXO41 is not established in the retrieved literature; the most robust mechanistic readouts relate to centrosomal targeting and cilia phenotypes rather than identified ubiquitination substrates."