Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
PEX12 interacts with PEX5 and PEX10 and acts downstream of receptor docking in peroxisomal matrix protein import.
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PEX12 zinc-binding domain binds PEX5 and PEX10
"the zinc-binding domain of PEX12 binds both PEX5, the PTS1 receptor, and PEX10, another integral peroxisomal membrane protein required for peroxisomal matrix protein import."
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S320F mutation reduces PEX12-PEX5 and PEX12-PEX10 interactions
"we identified a patient with a missense mutation in the PEX12 zinc-binding domain, S320F, and observed that this mutation reduces the binding of PEX12 to PEX5 and PEX10."
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PEX12 and PEX10 act downstream of receptor docking in import
"PEX12 and PEX10 play direct roles in peroxisomal matrix protein import downstream of the receptor docking event."
PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis.
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PEX19 binds PEX12 for membrane targeting
"PEX19 binds a broad spectrum of PMPs, displays saturable PMP binding, and interacts with regions of PMPs required for their targeting to peroxisomes."
Molecular anatomy of the peroxin Pex12p: ring finger domain is essential for Pex12p function and interacts with the peroxisome-targeting signal type 1-receptor Pex5p and a ring peroxin, Pex10p.
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RING finger of PEX12 binds PEX5 and PEX10
"The RING finger of Pex12p bound to Pex10p and the PTS1-receptor Pex5p."
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RING finger is essential for PEX12 function but not peroxisomal targeting
"RING finger is essential for peroxisome-restoring activity of Pex12p but not necessary for targeting to peroxisomes."
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PEX12 co-immunoprecipitates with PEX10
"Pex12p was co-immunoprecipitated with Pex10p from CHO-K1 cells"
Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay.
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PEX12 RING domain interacts with PEX5
"the C(3)HC(4) RING (really interesting new gene) finger domain of Pex12p does not alter the binding properties of Pex5p for the C-terminal peroxisome-targeting signal PTS1."
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PEX12 RING domain does not alter PEX5-PTS1 binding
"the C(3)HC(4) RING (really interesting new gene) finger domain of Pex12p does not alter the binding properties of Pex5p for the C-terminal peroxisome-targeting signal PTS1."
PEX5 binds the PTS1 independently of Hsp70 and the peroxin PEX12.
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PEX12 zinc RING domain has no effect on PEX5-PTS1 binding kinetics
"Another protein known to interact with the PTS1-binding domain of PEX5, the PEX12 zinc RING domain, also had no discernable effect on PEX5-PTS1 binding kinetics."
The peroxisomal membrane targeting elements of human peroxin 2 (PEX2).
A novel PEX12 mutation identified as the cause of a peroxisomal biogenesis disorder with mild clinical phenotype, mild biochemical abnormalities in fibroblasts and a mosaic catalase immunofluorescence pattern, even at 40 degrees C.
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R34S mutation causes mild PBD
"PEX12 sequencing revealed homozygosity for a novel c.102A>T (p.R34S) missense mutation affecting a partially conserved residue in the N-terminal region important for localization to peroxisomes."
PEX14 is required for microtubule-based peroxisome motility in human cells.
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PEX12 identified as constituent of PEX14 complexes at the peroxisomal membrane
"Using mass spectrometric analysis, almost all known human peroxins involved in protein import were identified as constituents of the PEX14 complexes."
Distinct modes of ubiquitination of peroxisome-targeting signal type 1 (PTS1) receptor Pex5p regulate PTS1 protein import.
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PEX10 has intrinsic E3 activity enhanced by PEX12
"The E3 activity of Pex10p is essential for its peroxisome-restoring activity, being enhanced by another RING peroxin, Pex12p."
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PEX12 does not have intrinsic E3 ubiquitin ligase activity
"RING finger Pex10p functions as an E3 with an E2, UbcH5C."
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PEX10-PEX12 complex monoubiquitinates PEX5 at lysine residues
"The Pex10p·Pex12p complex catalyzes monoubiquitination of Pex5p at one of multiple lysine residues in vitro, following the dissociation of Pex5p from Pex14p and the PTS1 cargo."
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RING peroxins required for both mono- and polyubiquitination of PEX5
"RING peroxins are required for both modes of Pex5p ubiquitination, thus playing a pivotal role in Pex5p shuttling."
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C304W mutation abolishes PEX12 ability to activate PEX10 E3 activity
"The E3 activity of Pex10p is essential for its peroxisome-restoring activity, being enhanced by another RING peroxin, Pex12p."
ATM functions at the peroxisome to induce pexophagy in response to ROS.
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PEX2/10/12 E3 ligase participates in ROS-induced PEX5 ubiquitination for pexophagy
"The RING peroxins PEX2, PEX10 and PEX12 are part of a peroxisome-localized E3 ligase responsible for polyubiquitination of PEX5"
A reference map of the human binary protein interactome.
A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.
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Cryo-EM structure of fungal PEX2-PEX10-PEX12 complex
"Each subunit of the complex contributes five transmembrane segments that co-assemble into an open channel."
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Each subunit contributes 5 TM segments forming a retrotranslocation channel
"Each subunit of the complex contributes five transmembrane segments that co-assemble into an open channel. The three ring finger domains form a cytosolic tower, with ring finger 2 (RF2) positioned above the channel pore."
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RF2 (PEX2) positioned above channel pore for monoubiquitination
"We propose that the N terminus of a recycling receptor is inserted from the peroxisomal lumen into the pore and monoubiquitylated by RF2 to enable extraction into the cytosol."
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RF10 and RF12 cooperate for polyubiquitination in RADAR pathway
"If recycling is compromised, receptors are polyubiquitylated by the concerted action of RF10 and RF12 and degraded."
Isolation of the human PEX12 gene, mutated in group 3 of the peroxisome biogenesis disorders.
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PEX12 identified as the gene mutated in CG3 PBD
"PEX12 expression restored peroxisomal protein import in fibroblasts from PBD patients of complement group 3 (CG3) and frameshift mutations in PEX12 were detected in two unrelated CG3 patients."
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PEX12 localizes to peroxisome membrane
"PEX12 shared the same subcellular distribution as yeast Pex12p and localized to the peroxisome membrane."
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PEX12 is essential for matrix protein import
"PEX12 expression restored peroxisomal protein import in fibroblasts from PBD patients of complement group 3 (CG3)"
PEX12 encodes an integral membrane protein of peroxisomes.
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PEX12 is an integral peroxisomal membrane protein
"PEX12 encodes an integral membrane protein of peroxisomes."
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C-terminal region exposed to cytoplasm
Peroxisome synthesis in the absence of preexisting peroxisomes.
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PEX12myc used as peroxisomal membrane protein marker
"The CG3 cell line, PBD097, is a compound heterozygote for two inactivating frameshift mutations in PEX12 (Chang et al., 1997)."
PEX2:PEX10:PEX12 binds PEX5S,L (in PEX5S:PEX13:PEX14) and Ub:UBE2D1,2,3
PEX2:PEX10:PEX12 monoubiquitinates PEX5S,L at cysteine-11
Cargo of PEX5S,L translocates from the cytosol to the peroxisomal matrix
PEX5S,L:Cargo binds PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Module)
PEX2:PEX10:PEX12 monoubiquitinates PEX5L at cysteine-11
PEX1:PEX6:PEX26:ZFAND6 dissociates Ub:PEX5L and PEX7 from PEX14:PEX13:PEX2:PEX10:PEX12 and translocates PEX5L and PEX7 from the peroxisomal membrane to the cytosol
Cargo of PEX5L:PEX7 translocates from the cytosol to the peroxisomal matrix
PEX2:PEX10:PEX12:Ub:PEX5L:PEX7:PEX13:PEX14 binds PEX1:PEX6:PEX26 and ZFAND6
PEX2:PEX10:PEX12 binds PEX5L (in PEX5L:PEX7:PEX13:PEX14:PEX2:PEX10:PEX12) and Ub:UBE2D1,2,3
PEX2:PEX10:PEX12:Ub:PEX5S,L:PEX13:PEX14 binds PEX1:PEX6:PEX26 and ZFAND6
PEX3:PEX19:class I PMP dissociates
PEX19:class I PMP binds PEX3
PEX19 binds class I peroxisomal membrane proteins
Deep research review of PEX12 function and biology