SPAC25B8.09 is a soluble methyltransferase type-11 protein found in the nucleus and cytosol. PomBase assigns trans-aconitate methyltransferase activity through curated orthology to budding-yeast Tmt1. This supports a probable small-molecule methyltransferase, while the predominant endogenous substrate and physiological role in fission yeast remain untested. Budding-yeast Tmt1 also methylates 3-isopropylmalate, so trans-aconitate detoxification is not the only plausible metabolic context.
The PomBase target page explicitly labels biological role inferred and provides TMT1/SGD:S000000977 orthology. The downloaded P32643 record verifies that identifier and its experimental sources. PMID:11695919(https://pubmed.ncbi.nlm.nih.gov/11695919/) establishes Tmt1 trans-aconitate methyltransferase activity; PMID:15147181(https://pubmed.ncbi.nlm.nih.gov/15147181/) identifies 3-isopropylmalate as its major endogenous substrate in yeast extracts. The two substrate observations are compatible; neither makes the other reaction false.
Curated orthology is reasonable support for target molecular capability. It does not settle the dominant physiological substrate, conservation of the starvation response, or a universal substrate for PTHR44942. Falcon misses both Tmt1 orthology and the target HDA nucleus/cytosol record. Its blanket “location unknown” is therefore not adopted; nor is absence of a target biochemical paper treated as grounds for erasing a curator-supported localization.
Exact retained output. These are name/location claims, not emitted GO/EC predictions. CNN denotes overlap with existing supported annotation; it does not establish literal membership in the training set.
| Claim type | Verbatim output | Assessment | Evidence and interpretation |
|---|---|---|---|
| name | Methyltransferase type 11 domain-containing protein | CNN | The claim specifies a domain class, not a methyl acceptor. IPR013216/PF08241 directly support that class. The curated Tmt1 orthology makes small-molecule methylation a reasonable additional inference but does not experimentally identify the target substrate. |
PMID:11695919(https://pubmed.ncbi.nlm.nih.gov/11695919/):
We have identified the yeast open reading frame YER175c as the gene encoding the
trans-aconitate methyltransferase of Saccharomyces cerevisiae.
PMID:15147181(https://pubmed.ncbi.nlm.nih.gov/15147181/):
the major
methylated endogenous product of Tmt1 in yeast extracts
The genuine Falcon report is retained with its provider metadata and artifact. Its conclusions were checked against the target record, exact accession and primary sources described above. Scientific uncertainties are recorded as UNC/UNDECIDED findings rather than a request for another reviewer to perform this assessment.