PMID:24811710

Genomic adaptations of the halophilic Dead Sea filamentous fungus Eurotium rubrum

Source of the CBS 135680 genome sequence for Eurotium rubrum (now Aspergillus ruber), providing the genomic context for the fog gene cluster.

"Genomic adaptations of the halophilic Dead Sea filamentous fungus Eurotium rubrum"

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

file:ASPRC/fogD/fogD-notes.md

fogD research notes

file:ASPRC/fogD/fogD-hypotheses/sdr-lipid-functions-and-polyketide-release/openscientist.md

OpenScientist focused report on FogD TreeGrafter lipid transfers and flavoglaucin reductase scope

The six Ayr1-derived TreeGrafter lipid terms are unsupported for FogD, whose direct evidence supports a flavoglaucin-cluster SDR role.

"No FogD-specific assay, localization experiment, or mutant supports any of these six terms."

PMID:32134669

Biosynthesis of the Prenylated Salicylaldehyde Flavoglaucin Requires Temporary Reduction to Salicyl Alcohol for Decoration before Reoxidation to the Final Product.

The pathway passes through alkylated salicyl alcohol intermediates before later decoration and reoxidation.

"The polyketide skeleton was released as alkylated salicyl alcohols"

PMID:24187129

Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae.

Ayr1 has experimentally supported triacylglycerol lipase activity in addition to its reductase assignment.

"These investigations led to the identification of Ayr1p as a novel triacylglycerol lipase of yeast lipid droplets"

file:ASPRC/fogD/fogD-uniprot.txt

UniProt record for FogD A0A017SE81

Curated record assigns FogD to the SDR family and proposes reductive release of modified FogA-bound products.

"involved in the reductive release of the modified PKS products"

GO_REF:0000118

TreeGrafter-generated GO annotations

Six source assertions are preserved. Historical PTN001211783 is absent from the current family PAINT slice, which records Ayr1-seeded IBDs for the six terms at PTN001213826 and PTN001963740. The correspondence to the historical target graft is not established.