PTHR19370 PAINT annotations
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PAINT node PTN000452207 propagates cytochrome-b5 reductase activity with MCR1 and PGA3 seeds, while node PTN000452208 propagates ergosterol biosynthetic process from a self-seeded S. cerevisiae MCR1 assertion.
"PTHR19370 PTN000452207 GO:0004128 F IBD false"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Biodiversity of the P450 catalytic cycle: yeast cytochrome b5/NADH cytochrome b5 reductase complex efficiently drives the entire sterol 14-demethylation (CYP51) reaction.
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Purified fungal sterol 14-demethylation can be supported by a cytochrome b5/NADH-cytochrome b5 reductase electron-transfer system.
"fungal CYP51 mediated sterol 14alpha-demethylation can be wholly and efficiently supported by the cytochrome b5/NADH cytochrome b5 reductase electron transport system"
Mitochondrial NADH-cytochrome b(5) reductase plays a crucial role in the reduction of D-erythroascorbyl free radical in Saccharomyces cerevisiae.
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MCR1 disruption nearly eliminated NADH-D-erythroascorbyl free-radical reductase activity and caused hypersensitivity to oxidants.
"the mcr1 disruptant cells were hypersensitive to hydrogen peroxide and menadione"
Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins.
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Highly purified S. cerevisiae mitochondrial outer membranes were profiled proteomically.
"We used a eukaryotic model organism, the yeast Saccharomyces cerevisiae, to determine the proteome of highly purified mitochondrial outer membranes."
Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins.
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The study generated a high-throughput in vitro interaction map for yeast WW domains, including Rsp5 WW domains.
"We used protein microarray technology to generate a protein interaction map for 12 of the 13 WW domains present in proteins of the yeast S. cerevisiae."
Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae.
Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.
The outer membrane form of the mitochondrial protein Mcr1 follows a TOM-independent membrane insertion pathway.
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The IMS and outer-membrane forms of Mcr1 use different biogenesis routes; the outer-membrane form inserts without the known TOM import components.
"while the IMS form crosses the outer membrane via the translocase of the outer mitochondrial membrane (TOM) complex, the other form is integrated into the outer membrane by a process that does not require any of the known import components at the outer membrane"
Intermembrane space proteome of yeast mitochondria.
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Soluble Mcr1 was used as an intermembrane-space marker in Bax-stimulated release assays of purified mitochondria.
"IMS proteins like cytochrome c or the soluble isoform of Mcr1 were largely released into the SN"
Quantitative variations of the mitochondrial proteome and phosphoproteome during fermentative and respiratory growth in Saccharomyces cerevisiae.
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The study quantified the yeast mitochondrial proteome and phosphoproteome in fermentative and respiratory growth.
"we performed an overall quantitative proteomic and phosphoproteomic study of isolated mitochondria"
Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification.
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Recombinant Mcr1 can reduce Dph3 more slowly than Cbr1, while Cbr1 is the major physiological reductase for Dph3.
"both Mcr1 and Ncp1 reduced Dph3 at a slower rate compared to that of Cbr1 under similar reaction conditions"
Incomplete arrest in the outer membrane sorts NADH-cytochrome b5 reductase to two different submitochondrial compartments.
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S. cerevisiae MCR1 encodes mitochondrial NADH-cytochrome b5 reductase isoforms partitioned between the outer membrane and intermembrane space.
"The S. cerevisiae gene MCR1 encodes two mitochondrial isoforms of NADH-cytochrome b5 reductase."
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Mcr1 uses incomplete outer-membrane translocation arrest to produce an outer-face p34 form and an intermembrane-space p32 form.
"This precursor protein is inserted into the outer membrane, but only about one-third of the molecules become firmly anchored to the outer face of that membrane."
SGD gene description for MCR1 (yeast)