Use of the ND evidence code for Gene Ontology (GO) terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Mmf1p Couples Amino Acid Metabolism to Mitochondrial DNA Maintenance in Saccharomyces cerevisiae.
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The RidA-family protein Mmf1p (the S. cerevisiae ortholog of mmf2) is an enamine/imine deaminase that deaminates 2-aminoacrylate, preempting metabolic stress and indirectly maintaining the mitochondrial genome.
"preempting metabolic stress and loss of the mitochondrial"
Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome.
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Mmf1p is a mitochondrial matrix factor whose deletion causes loss of mitochondrial DNA; the source of the "maintenance of mitochondrial function" (Mmf) name applied to mmf2.
"Mmf1p is a mitochondrial matrix"
Schizosaccharomyces pombe Pmf1p is structurally and functionally related to Mmf1p of Saccharomyces cerevisiae.
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S. pombe contains two p14.5/Rid-family homologs, Pmf1p and Hpm1p; the study experimentally localized and complementation-tested Pmf1p, not Hpm1p/mmf2.
"In this organism two p14.5 homologous proteins are present: Pmf1p (pombe mitochondrial factor 1) and Hpm1p (homologous Pmf1p factor 1)."
Genomic and experimental evidence for multiple metabolic functions in the RidA/YjgF/YER057c/UK114 (Rid) protein family.
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The Rid family comprises multiple subfamilies; RidA and the Rid1/Rid2/Rid3 subfamilies retain the conserved active-site arginine essential for imine/enamine deaminase activity.
"The Rid1, Rid2, and Rid3 subfamilies retain the conserved arginine and glutamate residues found in RidA"
Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA.
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Purified human RIDA, a eukaryotic RidA ortholog, directly deaminates reactive enamine-imine intermediates.
"We found that hRIDA was able to deaminate reactive enamine-imine intermediates"
Two novel fish paralogs provide insights into the Rid family of imine deaminases active in pre-empting enamine/imine metabolic damage.
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Two duplicated vertebrate RidA proteins both retained imine-deaminase activity, while differing markedly in substrate preference.
"Both proteins exhibited in vitro imine deaminase activity, but markedly different substrate specificity."
OpenScientist evaluation of the proposed specific RidA activity for mmf2
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The specific RidA term is supported as an inference from orthology, family placement, and conserved catalytic features, but not as direct experimental evidence on S. pombe mmf2.
"The seed hypothesis is **supported**"
UniProtKB record for S. pombe mmf2 (Q9UR06)
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UniProt assigns mmf2 to the PANTHER subfamily named PROTEIN HMF1-RELATED, which makes its relationship to the mitochondrial Mmf1p and cytosolic Hmf1p branches a curation question rather than an established orthology claim.
"DR PANTHER; PTHR11803:SF58; PROTEIN HMF1-RELATED; 1."