Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
A reference map of the human binary protein interactome.
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HuRI is a systematic human binary protein-interaction map generated by repeated Y2H screening.
"To map the reference interactome, we performed nine screens of Space III"
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The reported pairs underwent pairwise retesting and sequence confirmation.
"followed by pairwise verification by quadruplicate retesting and sequence confirmation"
Characterization of ITIH6, a novel inter-α-inhibitor family member with prognostic significance in cholangiocarcinoma.
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The study describes ITIH6 as previously uncharacterized.
"a previously uncharacterized member of the ITI heavy chain (ITIH) family"
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ITIH6 has canonical VIT/vWA domains and a uniquely expanded central region.
"ITIH6 contains the two canonical domains characteristic of ITIH proteins (VIT, vWA), but also harbors a large central region"
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ITIH6 protein has a tissue-restricted glandular expression pattern.
"abundant expression in salivary glands, kidney, colon, and stomach"
Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan.
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Human inter-alpha-inhibitor contains one common inhibitory chain.
"each protein contained a single, identical, trypsin-inhibitory chain of 30,000 Da"
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The inter-alpha-inhibitor heavy chains are noninhibitory.
"Inter-alpha-trypsin inhibitor contains noninhibitory heavy chains of 65,000 and 70,000 Da"
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
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The initiative isolated full-length cDNAs for candidate secreted and transmembrane proteins.
"The isolation of full-length cDNA clones for each of these candidate genes resulted in the identification of >1000 novel proteins."
UniProtKB record for human ITIH6 (Q6UXX5)
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ITIH6 is a reviewed 1,313-amino-acid precursor.
"Reviewed; 1313 AA."
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UniProt predicts an N-terminal signal peptide.
"FT SIGNAL 1..23"
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UniProt assigns VIT and vWA domains.
"FT DOMAIN 24..150"
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UniProt predicts secretion by similarity.
"CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}."
Manual literature and annotation review notes for human ITIH6
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ITIH6 has no demonstrated intrinsic protease-inhibitor activity.
"ITIH6 has no Kunitz/bikunin inhibitor domain and no direct protease-inhibition assay."
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Hyaluronan transfer remains unverified despite a plausible cleavage motif.
"The conserved candidate cleavage motif makes a canonical heavy-chain route plausible"
Manual literature synthesis for human ITIH6 (Q6UXX5)
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ITIH6's specific molecular activity and process remain unknown.
"Its specific molecular activity and biological process are still unknown."
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No direct experiment establishes a bikunin-linked, HA-transferable ITIH6 heavy chain.
"No experiment in the 2025 paper demonstrates ITIH6 secretion, incorporation into a bikunin-containing complex, covalent linkage to chondroitin sulfate, transfer to hyaluronan, or hyaluronan binding."