Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Evolutionary analyses of ABC transporters of Dictyostelium discoideum.
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Some Dictyostelium ABC superfamily members serve specialized developmental roles by releasing intercellular signals at appropriate developmental stages.
"Other members of the superfamily may serve specialized developmental roles by releasing intercellular signals at appropriate stages of development"
Peptide signaling during terminal differentiation of Dictyostelium.
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Production of SDF-2 depends on the prestalk gene tagC, which encodes an ABC transporter fused to a serine protease.
"Production of SDF-2 depends on expression of the prestalk gene, tagC , which encodes a member of the ABC transporter family fused to a serine protease"
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tagC-null cells cannot process exogenously added AcbA into SDF-2, demonstrating that TagC performs the AcbA-processing step.
"primed KP cells carrying a null mutation in tagC were unable to process exogenously added AcbA into SDF-2 activity"
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AcbA processing to SDF-2 is blocked by a serine protease inhibitor, indicating a serine protease activity.
"Addition of the serine protease inhibitor tosyl phenylalanyl chloromethylketone to primed cells just before addition of AcbA blocked all production of SDF-2"
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AcbA released from prespore cells is proteolytically cleaved by prestalk cells to generate SDF-2, triggering rapid sporulation.
"It appears that AcbA is released from prespore cells and proteolytically cleaved by prestalk cells to generate SDF-2, which then triggers rapid sporulation of prespore cells"
GABA induces terminal differentiation of Dictyostelium through a GABAB receptor.
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GABA induces exposure of the TagC protease domain on the surface of prestalk cells, where it converts AcbA to SDF-2.
"GABA also induces exposure of the protease domain of TagC on the surface of prestalk cells where it can convert AcbA to SDF-2"
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Prespore cells rapidly encapsulate into spores in response to the SDF-2 signal generated by TagC.
"they rapidly encapsulate in response to the signalling peptide SDF-2"
A multidrug resistance transporter/serine protease gene is required for prestalk specialization in Dictyostelium.
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The tag genes encode MDR-transporter/serine-protease proteins whose mechanism is inferred to include proteolysis and export of peptide signals.
"implying a mechanism of action that includes"
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Prestalk cells induce encapsulation of prespore cells during culmination.
"prestalk cells induce encapsulation of prespore cells"