Gene Ontology annotation through association of InterPro records with GO terms

InterPro2GO assigns generic sulfotransferase activity from the sulfotransferase domain; correct but unspecific.

Annotation inferences using phylogenetic trees

PAINT IBA assigns cytoplasm and sulfation from the broad cytosolic sulfotransferase node and aromatic desulfoglucosinolate sulfotransferase activity from the SOT16/17/18 clade node; all consistent with experimental data.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt subcellular location mapping to cytoplasm, derived from GFP localization data.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Rhea/EC-based IEA assigns aliphatic desulfoglucosinolate sulfotransferase activity (EC 2.8.2.38), consistent with kinetic data.

GO_REF:0000122

AtSubP analysis

AtSubP predicts cytoplasmic localization.

PMID:15358770

Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure.

AtST5a/b/c (SOT16/18/17) catalyze sulfation of desulfoglucosinolates, the final step of core glucosinolate biosynthesis; ST5b and ST5c prefer long-chain methionine-derived substrates.

"long chain dsGSs derived from methionine are the preferred substrates of AtST5b and AtST5c"

PMID:16367753

The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed.

AtSOT16-18 are PAPS-dependent desulfoglucosinolate sulfotransferases with distinct kinetics, are cytoplasmic by GFP fusion, and are differentially expressed.

"All three desulfoglucosinolate AtSOT proteins are localized in the cytoplasm"

PMID:19077143

Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana.

Kinetic comparison of AtSOT16-18 from C24 (and SOT18 from Col-0) showing distinct substrate preferences.

"AtSOT18 preferred the long-chain ds-Gl, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine"