Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Exocytosis of platelet dense granule content
Isolation of dense granules from human platelets.
The human plasma proteome: a nonredundant list developed by combination of four separate sources.
TSG-6 potentiates the antitissue kallikrein activity of inter-alpha-inhibitor through bikunin release.
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Free bikunin, not HC-associated bikunin, supplies relevant protease inhibition.
"bikunin in its free state, but not when associated with HCs, is a relevant protease inhibitor"
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TSG-6 transfers heavy chains from inter-alpha-inhibitor and pre-alpha-inhibitor onto HA.
"TSG-6 mediates the transfer of the heavy chains from IαI/PαI onto HA"
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Human airway epithelial cells express HC3 and bikunin as pre-alpha-inhibitor components.
"bikunin and HC3 (i.e., pre-alpha-inhibitor) were also induced by TNF-alpha"
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
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The HDA source analyzed pooled expressed-prostatic-secretion urine exosome preparations.
"samples, ~900 proteins were detected."
Human plasma inter-alpha-trypsin inhibitor is encoded by four genes on three chromosomes.
Unmasking a hyaluronan-binding site of the BX(7)B type in the H3 heavy chain of the inter-alpha-inhibitor family.
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A proteolytically generated N-terminal fragment of purified human HC3 binds HA.
"thermolysin-generated, N-terminal fragment of this H3 chain strongly bound HA"
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Intact mature HC3 did not bind HA in the same assay.
"whereas the intact H3 chain did not."
The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2.
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Purified human plasma components form HC3-TSG-6 and then HC3-HA complexes.
"the formation of HC3.TSG-6 and subsequently HC3.hyaluronan complexes."
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Both TSG-6 and HC2 are required for HC3 transfer.
"both HC2 and TSG-6 are required for the transesterification reactions to occur."
Human pre-alpha-inhibitor: isolation from a by-product of industrial scale plasma fractionation and structural analysis of its H3 heavy chain.
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Human pre-alpha-inhibitor contains bikunin as its antiprotease component and HC3 as the covalently linked heavy chain.
"plasma. It comprises bikunin (BK) responsible for antiprotease activity,"
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HC3 is covalently linked to bikunin in pre-alpha-inhibitor.
"covalently linked to a heavy chain H3."
Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan.
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Inter-alpha-inhibitor-family complexes contain a distinct 30-kDa inhibitory chain.
"trypsin-inhibitory chain of 30,000 Da"
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Pre-alpha-inhibitor contains a separate 90-kDa heavy chain.
"pre-alpha-trypsin inhibitor contains a heavy chain of 90,000"
Chondroitin 4-sulfate covalently cross-links the chains of the human blood protein pre-alpha-inhibitor.
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Human pre-alpha-inhibitor contains one heavy and one light chain linked by a protein-glycosaminoglycan-protein cross-link.
"protein-glycosaminoglycan-protein (PGP) cross-link."
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The heavy-chain C-terminal Asp is esterified to the chondroitin-sulfate glycan.
"esterified, via the alpha-carbon of its C-terminal Asp, to C-6 of an internal"
Equivalent involvement of inter-alpha-trypsin inhibitor heavy chain isoforms in forming covalent complexes with hyaluronan.
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Mouse HC3 occurs in physiological, pathological, and in-vitro SHAP-HA complexes.
"All three HC isoforms are found in the SHAP-hyaluronan"
UniProtKB record for human ITIH3 (Q06033)
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ITIH3 is a reviewed secreted precursor.
"SUBCELLULAR LOCATION: Secreted."
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HC3 and bikunin constitute pre-alpha-inhibitor.
"P-alpha-I) is composed of ITIH3/HC3 and bikunin."
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HC3 is ester-linked through bikunin chondroitin sulfate after processing.
"Heavy chains are linked to bikunin via chondroitin 4-sulfate"
Manual literature and annotation review notes for human ITIH3
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HC3 is not the protease-inhibitor component of pre-alpha-inhibitor.
"ITIH3/HC3 must therefore not inherit the protease-inhibitor activity of bikunin or of the named complex."
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HC3 transfer to HA is direct, whereas HC2 and TSG-6 provide the transfer machinery.
"ITIH3 is the transferred substrate/structural heavy chain; TSG-6 and HC2 provide the transfer machinery."
Manual literature synthesis for human ITIH3 (Q06033)
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HC3 is a transferable HA-associated heavy chain, not an inhibitor or transferase.
"HC3 is therefore a transferable HA-associated heavy chain, while TSG-6 and HC2 supply the transfer mechanism."