UniProtKB entry Q6QHF9 (PAOX_HUMAN)
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion.
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Human PAOX cDNA expressed in HEK-293 cells confers rapid oxidation of N1-acetylspermine to spermidine, with substrate ranking N1-acetylspermine = N1-acetylspermidine > N1,N12-diacetylspermine >> spermine; spermidine is not a substrate. This is distinct from spermine oxidase (SMO), which prefers free spermine.
"a preference ranking of N1-acetylspermine= N1-acetylspermidine> N1,N12-diacetylspermine>>spermine; spermidine was not acted upon"
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PAOX overexpression shifts intracellular polyamine pools, decreasing spermine and increasing spermidine, supporting its role in polyamine back-conversion and homeostasis.
"intracellular spermine pools decreased by approx. 30%, whereas spermidine increased 2-4-fold"
Guide molecule-driven stereospecific degradation of alpha-methylpolyamines by polyamine oxidase.
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FAD-dependent polyamine oxidase (PAO) is a key enzyme in spermidine/spermine catabolism whose natural substrates are N1-acetylspermidine, N1-acetylspermine and N1,N12-diacetylspermine.
"the catabolism of polyamines spermidine and spermine. The natural substrates for"
PAO:FAD oxidises NASPM to PTCN
PAOX:FAD oxidises NASPN to SPM
Cargo of PEX5S,L translocates from the cytosol to the peroxisomal matrix
PEX5S,L:Cargo binds PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Module)