Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Arginine Biosynthesis Modulates Pyoverdine Production and Release in Pseudomonas putida as Part of the Mechanism of Adaptation to Oxidative Stress.
Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
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Comparative Pseudomonas genetics identifies AruC as the AST-pathway succinylornithine aminotransferase.
"The aruR, aruC, aruD, aruB, and aruE genes specify the ArgR regulatory protein, N2-succinylornithine 5-aminotransferase, N-succinylglutamate 5-semialdehyde dehydrogenase, N2-succinylarginine dihydrolase, and N-succinylglutamate desuccinylase, respectively"
Determination of the structure of the catabolic N-succinylornithine transaminase (AstC) from Escherichia coli.
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Defines the PLP-dependent succinylornithine transamination chemistry and demonstrates substrate-bound AstC structure.
"AstC is a dimeric, pyridoxal phosphate (PLP)-dependent δ–aminotransferase that operates a ping-pong bi-bi reaction mechanism with succinylornithine (SO) and α-ketoglutarate (αKG) as co-substrates and succinylglutamic semialdehyde and glutamate as co-products"
UniProtKB entry for PSEPK ArgD
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Assigns P59319 to the PLP-dependent ACOAT reaction while noting broader family activity.
"Full=Acetylornithine aminotransferase"
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Notes a possible additional lysine-pathway activity that remains an inference.
"May also have succinyldiaminopimelate aminotransferase"
QuickGO annotation snapshot for PSEPK ArgD
OpenScientist literature and database synthesis for PSEPK PP_4481
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Integrates genomic context, orthology, and family evidence to identify PP_4481 as the AST-pathway succinylornithine transaminase while retaining possible secondary ACOAT activity.
"the **primary physiological role of PP_4481 is catabolic**: it is the **N²-succinyl-L-ornithine 5-aminotransferase (AstC/AruC, EC 2.6.1.81)**"
OpenScientist module-level synthesis for PSEPK arginine biosynthesis
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Identifies P59319 as a promiscuous, non-core contributor to the biosynthetic transamination step rather than a dedicated biosynthetic ArgD.
"This is a genuine lineage-specific alternative, not an over-annotation to discard"
PANTHER PTHR11986 member snapshot
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Places P59319 in the AstC-like succinylornithine-transaminase subfamily.
"P59319,Acetylornithine aminotransferase,protein,160488,Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440),Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440),argD,406,PTHR11986:SF113,SUCCINYLORNITHINE TRANSAMINASE,True"
PSEPK UniProt metadata-derived gene list
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Shows PP_4481 adjacent to astA-I, astA-II, astB, and astD in the arginine succinyltransferase catabolic locus.