Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma.
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PINT87aa is translated via an IRES from circPINTexon2, a circRNA formed by back-splicing of LINC-PINT exon 2, and the endogenous peptide is detected by IP-MS.
"Endogenous immunoprecipitation using this antibody followed by LC-MS/MS in 293T cells further confirmed that the 10-kDa peptide sequences matched the predicted 87-aa sORF"
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The coding circRNA is cytoplasmic whereas the linear lncRNA is nuclear.
"linear LINC-PINT largely localized to the nucleus, whereas circPINTexon2 was mostly cytoplasmic"
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PINT87aa directly binds PAF1 and co-occupies the CPEB1 promoter with it; it is proposed to anchor PAF1 complex at promoters and pause Pol II elongation.
"We assumed that PINT87aa may work as an anchor and keep PAF1 complex on target genes’ promoter, which sequentially pauses Pol II-induced mRNA elongation"
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PINT87aa does not bind DNA itself.
"Although no evidence showed that PINT87aa directly binds to DNA and act as a transcription factor"
Cellular senescence in hepatocellular carcinoma induced by a long non-coding RNA-encoded peptide PINT87aa by blocking FOXM1-mediated PHB2.
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PINT87aa binds the DNA-binding domain of FOXM1 and reduces FOXM1-driven PHB2 transcription, leading to reduced mitophagy and senescence of HCC cells when overexpressed.
"We confirmed that FOXM1 could promote the transcription of PHB2 and a negative regulatory effect of PINT87aa on PHB2 expression"
PINTology: A short history of the lncRNA LINC-PINT in different diseases.