TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry C5CNX5 (FETP_VARPS): Probable Fe(2+)-trafficking protein
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Vapar_2982 (C5CNX5) is a 90-residue predicted cytosolic YggX-family Fe(2+)-trafficking protein (HAMAP MF_00686, InterPro IPR007457) in Variovorax paradoxus S110.
"DR HAMAP; MF_00686; Fe_traffic_YggX; 1."
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HAMAP rule MF_00686 predicts that Vapar_2982 acts as a mediator in intracellular iron transactions between iron acquisition and Fe-S cluster synthesis or repair.
"CC -!- FUNCTION: Could be a mediator in iron transactions between iron
CC acquisition and iron-requiring processes, such as synthesis and/or
CC repair of Fe-S clusters in biosynthetic enzymes. {ECO:0000255|HAMAP-
CC Rule:MF_00686}."
Complete genome sequence of the metabolically versatile plant growth-promoting endophyte Variovorax paradoxus S110.
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The complete genome sequence of Variovorax paradoxus S110 comprises 6,754,997 bp with 6,279 predicted protein-coding sequences across two circular chromosomes.
"The complete genome sequence of V. paradoxus S110 is composed of 6,754,997 bp with 6,279 predicted protein-coding sequences within two circular chromosomes."
The YggX protein of Salmonella enterica is involved in Fe(II) trafficking and minimizes the DNA damage caused by hydroxyl radicals: residue CYS-7 is essential for YggX function.
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Demonstrates that bacterial YggX chelates/traffics intracellular Fe(II) via conserved Cys7 and protects DNA from Fenton-mediated hydroxyl radical oxidative damage.
"The ability of purified YggX to protect
DNA from Fenton chemistry mediated damage in vitro and to decrease the
concentration of Fe(II) ions in solution available for chelation provided a
framework for the interpretation of data obtained from in vivo experiments."
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A C7S substitution in Salmonella enterica YggX abolishes its function both in vivo and in vitro, establishing Cys7 as essential for Fe(II) trafficking.
"The
interpretation of in vitro assay results, within the context of the in vivo
phenotypes, was validated by a mutant variant of YggX (C7S) that was unable to
function in vivo or in vitro."
Protection from superoxide damage associated with an increased level of the YggX protein in Salmonella enterica.
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Shows that YggX increases cellular resistance to superoxide stress and protects labile [4Fe-4S] clusters from oxidative inactivation.
"Here it is shown that elevated levels of the YggX
protein increase the resistance of Salmonella enterica to superoxide stress,
reverse enzymatic defects attributed to oxidized [Fe-S] clusters, and decrease
the spontaneous mutation frequency."