Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunit IV.
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Identifies COX4I1 as subunit IV of cytochrome c oxidase (EC 1.9.3.1) and maps the gene to chromosome 16q22-qter.
"Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunit IV."
A novel mitochondrial protein DIP mediates E2F1-induced apoptosis independently of p53.
Knockdown of human COX17 affects assembly and supramolecular organization of cytochrome c oxidase.
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COX17 knockdown affects CIV assembly and supercomplex organization; COX4I1 observed in assembly intermediates.
"Knockdown of human COX17 affects assembly and supramolecular organization of cytochrome c oxidase."
Defining the membrane proteome of NK cells.
Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
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Identified COX4I1 in the mitochondrial phosphoproteome from human skeletal muscle.
"Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes."
Protein phosphorylation and prevention of cytochrome oxidase inhibition by ATP: coupled mechanisms of energy metabolism regulation.
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Demonstrated that PKA-dependent phosphorylation of Ser58 on COX4-1 prevents allosteric ATP inhibition of Complex IV, coupling cAMP signaling to mitochondrial respiration regulation.
"Protein phosphorylation and prevention of cytochrome oxidase inhibition by ATP: coupled mechanisms of energy metabolism regulation."
MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation.
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Identified COX4I1 in MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase) complexes, linking protein import to CIV assembly.
"MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation."
A proteome-scale map of the human interactome network.
MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly.
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COX4I1 found in MITRAC complexes during CIV assembly; MITRAC7 acts as COX1-specific chaperone in the assembly pathway.
"MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly."
Functional expression of choline transporter like-protein 1 (CTL1) and CTL2 in human brain microvascular endothelial cells.
The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits.
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LACE1/AFG1L physically interacts with COX4I1 and COX5A and mediates their degradation as part of mitochondrial protein quality control.
"The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits."
Architecture of the human interactome defines protein communities and disease networks.
Mutation in the COX4I1 gene is associated with short stature, poor weight gain and increased chromosomal breaks, simulating Fanconi anemia.
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Homozygous COX4I1 K101N variant causes Complex IV deficiency with growth failure and chromosomal instability. Functional rescue with wild-type COX4I1 restored COX activity.
"Mutation in the COX4I1 gene is associated with short stature, poor weight gain and increased chromosomal breaks, simulating Fanconi anemia."
Structure of the intact 14-subunit human cytochrome c oxidase.
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Cryo-EM structure (3.3A) of human CIV as part of supercomplex, confirming 14-subunit monomer composition including COX4I1. COX4I1 resolved with transmembrane helix, matrix domain, and IMS domain.
"Structure of the intact 14-subunit human cytochrome c oxidase."
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
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Study of how genetic variants affect protein interactions; COX4I1-SDCBP interaction tested.
"Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations."
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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Neurodegenerative disease interactome mapping; COX4I1-KLF11 interaction reported.
"Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Electron transfer from reduced cytochrome c to molecular oxygen
CO binds to Cytochrome c oxidase
TIMM21 carries COX4, COX5A, COX6C to MT-CO1:MITRAC
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COX4I1 is transported by TIMM21 to MT-CO1:MITRAC assembly intermediate.
"TIMM21 carries COX4, COX5A, COX6C to MT-CO1:MITRAC"
Metallochaperone inserts Cu2+ into MT-CO1
MT-CO1 and MT-CO2 complexes associate, installing heme moieties
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COX4I1 present during MT-CO1/MT-CO2 module joining in CIV assembly.
"MT-CO1 and MT-CO2 complexes associate, installing heme moieties"
MT-CO3, COX6A,B,7A and NDUFA4 bind to holo-MT-CO1,2 complex
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COX4I1 already incorporated during late CIV assembly steps.
"MT-CO3, COX6A,B,7A and NDUFA4 bind to holo-MT-CO1,2 complex"