Annotation inferences using phylogenetic trees

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:21531806

Binding and spreading of ParB on DNA determine its biological function in Pseudomonas aeruginosa.

ParB variants that bind DNA but cannot oligomerize or spread phenocopy parB-null mutants.

"indicating that the ability to spread is vital for ParB function in the cell"

PMID:15466051

ParB of Pseudomonas aeruginosa: interactions with its partner ParA and its target parS and specific effects on bacterial growth.

Purified ParB binds parS specifically.

"Purified ParB binds specifically to DNA containing a putative parS sequence"
ParB overproduction silences genes near parS.

"The overproduction of ParB was shown to inhibit the function of genes placed near parS."

PMID:19332810

ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters.

parB mutants produce anucleate cells and have growth and motility defects.

"All parB mutations affected both bacterial growth and swarming and swimming motilities, and increased the production of anucleate cells."

PMID:22322962

Identification of C-terminal hydrophobic residues important for dimerization and all known functions of ParB of Pseudomonas aeruginosa.

Dimerization underlies all known ParB properties.

"Thus so far all the properties of ParB seem to depend on dimerization."

PMID:25794281

A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa.

ParB binds all ten parS sites.

"It was found that ParB binds specifically to all ten parS sites, although with different affinities."

PMID:27820816

Regional Control of Chromosome Segregation in Pseudomonas aeruginosa.

ParB binds four oriC-proximal parS sites in vivo and forms a nucleoprotein complex.

"we show that ParB binds to four parS site located within 15 kb of oriC in vivo"

PMID:29648658

Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif.

ParB binds hundreds of genomic sites containing a half-parS motif.

"ChIP-seq analysis revealed 420 ParB-associated regions in wild-type strain"

PMID:28732084

Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sites parS3 and parS4 in Pseudomonas aeruginosa.

ParB bound at parS3/parS4 represses adjacent promoters.

"indicating that direct ParB- parS3/parS4 interactions repress the transcription in this region"

PMID:38843873

Inhibitor of Chromosome Segregation in Pseudomonas aeruginosa from Fungal Extracts.

Purified P. aeruginosa ParB hydrolyses CTP in a parS-stimulated manner.

"We found that the same holds true for the P. aeruginosa ParB."

PMID:36622167

Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.

The ParB N-terminal motif is required for interaction with ParA.

"showing that the N-terminal motif of ParB is required for these interactions"

PMID:31649139

Self-organization of parS centromeres by the ParB CTP hydrolase.

ParB is a parS-stimulated CTP hydrolase.

"We show that ParB is an enzyme that hydrolyzes cytidine triphosphate (CTP) to cytidine diphosphate (CDP)."

PMID:11849535

The parAB gene products of Pseudomonas putida exhibit partition activity in both P. putida and Escherichia coli.