PIWIL4 review notes

• Deep research: SKIPPED (falcon times out in this environment; perplexity-lite not installed). No deep-research file was generated or fabricated.
• Evidence sources: cached publications (just fetch-gene-pmids human PIWIL4), the UniProt record (PIWIL4-uniprot.txt), and additional mouse/fly
  primary papers fetched with just fetch-pmid (e.g. PMID:18922463, PMID:22020280, PMID:29437694, PMID:20011505, PMID:22902557).
• Reviewed as an exemplar for a generalized animal piRNA silencing module.

Key points

• Nuclear, slicer-dispensable effector directing de novo TE DNA methylation PMID:22020280.
• In somatic cancer cells Hiwi2 is mostly cytoplasmic and binds tRNA-derived piRNAs PMID:25038252.
• RNA endonuclease IBA marked over-annotated (FUNCTIONAL_DIVERGENCE); NOT-endonuclease ISS accepted. The validator's same-term consistency warning is expected (positive vs NOT row).