DKC1 primary-source locators

These are authored source notes, separate from the immutable publication caches. They distinguish independently inspected originals from cache availability. They are not additional experimental observations.

• PMID:22658674(https://doi.org/10.1016/j.cell.2012.04.031), Results “In Vivo Capture of HeLa RBPs” and Table S1 (original supplementary workbook): sheet HeLa mRNA interactome.txt, row 401 beneath the header, has ENSG00000130826/DKC1 classified as mRNA-interactome, crosslinked identified, control not identified. Adjusted DESeq and ion-count values are respectively 0.000015715649994767 and 0.00161953790274024. The study uses HeLa UV crosslinking, oligo(dT) capture and stringent washes. The numerical table is not an affinity or isoform-specific measurement.
• PMID:18358808(https://pmc.ncbi.nlm.nih.gov/articles/PMC2291539/), Results and protein-binding Methods: RNase-resistant dyskerin–RUVBL1 association is supplemented by recombinant MBP-dyskerin/pontin binding. This supplies direct pair evidence beyond the later AP-MS observations.
• PMID:18082603(https://pmc.ncbi.nlm.nih.gov/articles/PMC2917595/), PMID:20351177(https://pmc.ncbi.nlm.nih.gov/articles/PMC2876521/) and PMID:22527283(https://pmc.ncbi.nlm.nih.gov/articles/PMC3434483/): selected human purification/assembly Results and Methods were inspected independently. hTR association, two-copy H/ACA organization and BIO/ACA-driven RNA recruitment concern RNP assemblies, not purified isolated dyskerin affinities. Cache availability remains unchanged.
• PMID:19179534(https://pmc.ncbi.nlm.nih.gov/articles/PMC2728071/), Results on dyskerin purification and RNase treatment: NAF1/TCAB1/pontin associations and H/ACA RNA recovery support distinct assembly and mature RNP contexts. The linked supplementary endpoint did not produce an accessible scientific PDF.
• PMID:35271311(https://doi.org/10.1126/science.abi6983), Methods and OpenCell downloadable interaction table: exact DKC1-bait/NAF1-prey pair. Human endogenous CRISPR tags and triplicate affinity capture establish co-complex association, not binary binding or a uniquely resolved NAF1 isoform.
• PMID:33961781(https://pmc.ncbi.nlm.nih.gov/articles/PMC8165030/), STAR Methods: human ORFeome constructs, C-terminal FLAG/HA tags, HEK293T/HCT116 AP-MS and network aggregation. Source-specific DKC1–NAF1/SHQ1 and RUVBL1–DKC1 records were joined by pair plus publication, because one spoke-expanded interaction identifier can name several pairs.
• PMID:25467444(https://pmc.ncbi.nlm.nih.gov/articles/PMC4329143/), main Results/Methods: DKC1 depletion is distinguished from TRiC/TCAB1 misfolding. The DKC1-specific scaRNA and TCAB1-protein localization supplemental observations were not recovered; no claim of their absence is made.

A short original-only anchor for the direct pair is “Recombinant pontin bound recombinant MBP-dyskerin” (PMID:18358808, Results describing Figure 5E). This quote is kept here because the normal publication cache is abstract-only. The full paragraph distinguishes purified bacterial proteins from the preceding rabbit-reticulocyte association experiment.