Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database
Combined Automated Annotation using Multiple IEA Methods
A method of utrophin up-regulation through RNAi-mediated knockdown of the transcription factor EN1.
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EN1 binds the human UTRN promoter and represses UTRN expression.
"The findings suggest that EN1 directly interacts with the UTRN promoter. Small interfering RNA was used to inhibit EN1 gene expression. Higher utrophin mRNA levels were observed in EN1-inhibited cells compared with controls."
Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of dopaminergic neurons.
Wnt signaling in midbrain dopaminergic neuron development and regenerative medicine for Parkinson's disease.
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
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The study systematically measured methylation-sensitive sequence specificity for hundreds of human transcription factors.
"By analysis of 542 human TFs with methylation-sensitive SELEX (systematic evolution of ligands by exponential enrichment), we found that there are also many TFs that prefer CpG-methylated sequences."
Regional assignment of the human homeobox-containing gene EN1 to chromosome 2q13-q21.
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EN1 was mapped to human chromosome 2q13-q21.
"Here, we have refined the localization of EN1 to human chromosome 2q13-q21 using a mapping panel of rodent/human cell hybrids"
The mouse Engrailed-1 gene and ventral limb patterning.
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Mouse En1 is required for normal ventral limb patterning.
"Loss of Engrailed-1 function in mice results in dorsal transformations of ventral paw structures, and in subtle alterations along the proximal-distal limb axis."
The Engrailed homeobox genes determine the different foliation patterns in the vermis and hemispheres of the mammalian cerebellum.
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En1/En2 regulate regional cerebellar foliation after cerebellar cell specification.
"Furthermore, En1/En2 continue to regulate foliation after embryonic day 14, at which time Fgf8 isthmic organizer activity is complete and the major output cells of the cerebellar cortex have been specified."
Engrailed 1 shapes the dopaminergic and serotonergic landscape through proper isthmic organizer maintenance and function.
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En1 maintains the isthmic organizer and represses Otx2, Wnt1, and canonical Wnt signaling in rostral hindbrain.
"Our work suggests a newly-discovered role for En1: the repression of Otx2, Wnt1 and canonical Wnt-signaling in R1. Overall, our results suggest that En1 is essential for proper IsO maintenance and function."
Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1.
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Reduced En1 dosage causes progressive dopaminergic-neuron loss in adult mice.
"These loss and gain of function experiments firmly establish that En1/2 is a true survival factor for DA neurons in vivo."
Spatially restricted and developmentally dynamic expression of engrailed genes in multiple cerebellar cell types.
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En1 is expressed in several developing and postnatal cerebellar neuronal populations.
"DCN neurons and Purkinje cells continue to express En1 at P21, whereas En2 is mainly expressed in granule cells; both En1 and En2 continue to be expressed in mature cerebellar interneurons."
Non-coding deletions identify Maenli lncRNA as a limb-specific En1 regulator.
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Upstream deletions that abolish limb EN1 expression produce a conserved dorsoventral limb phenotype.
"Re-engineering of the human deletions in mice resulted in a complete loss of En1 expression in the limb and a double dorsal-limb phenotype that recapitulates the human disease phenotype."
Engrailed transcription factors direct excitatory cerebellar neuron diversity and survival.
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En1/En2 jointly regulate differentiation and survival across cerebellar excitatory-neuron lineages.
"We further reveal a similar function for EN1/2 in mediating TBR2 expression, neuron differentiation and survival in the other excitatory neurons (granule and unipolar brush cells)."