Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
In vitro processing of the human alkyl-dihydroxyacetonephosphate synthase precursor.
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Human precursor enters purified peroxisomes and is processed there; processing does not increase enzyme activity.
"exogenously added pre-alkyl-dihydroxyacetonephosphate synthase was imported and processed in purified peroxisomes in vitro."
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The precursor is already catalytically active before removal of its targeting presequence.
"Processing of alkyl-dihydroxyacetonephosphate synthase did not increase the activity of the enzyme."
Defining the membrane proteome of NK cells.
Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
PEX14 is required for microtubule-based peroxisome motility in human cells.
A proteome-scale map of the human interactome network.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Ether lipid synthesis: purification and identification of alkyl dihydroxyacetone phosphate synthase from guinea-pig liver.
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Native guinea-pig AGPS was purified as an ether-bond-forming enzyme.
"Alkyl-dihydroxyacetone phosphate synthase, the second enzyme involved in ether phospholipid biosynthesis from dihydroxyacetone phosphate and responsible for glycero-ether bond formation, has been purified from guinea-pig liver."
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Guinea-pig AGPS was extracted from a peroxisome-enriched membrane fraction.
"Alkyl-dihydroxyacetone phosphate synthase was solubilized from a membrane fraction prepared from an enriched peroxisome fraction with Triton X-100 and potassium chloride."
Alkyl-dihydroxyacetonephosphate synthase. Fate in peroxisome biogenesis disorders and identification of the point mutation underlying a single enzyme deficiency.
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Human R419H AGPS lacks the activity retained by a recombinant wild-type control.
"Expression of a recombinant protein carrying this mutation in Escherichia coli yielded an inactive enzyme, whereas a comparable control recombinant enzyme was active, providing further proof that this substitution is responsible for the inactivity of the enzyme and the phenotype."
1-palmitoylglycerone phosphate + hexadecanol => O-hexadecylglycerone phosphate + palmitate
palmitoyl-CoA + DHAP => 1-palmitoylglycerone phosphate + CoASH
PEX7 binds cargo proteins containing PTS2
Cargo of PEX5L:PEX7 translocates from the cytosol to the peroxisomal matrix
UniProt O00116: Alkyldihydroxyacetonephosphate synthase, peroxisomal
Precursor of ether phospholipids is synthesized by a flavoenzyme through covalent catalysis.
Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.