Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana.
Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis.
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana.
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AtLrgB mutants have interveinal chlorotic and premature necrotic leaves; the authors proposed a role in chloroplast development but no molecular mechanism.
"T-DNA insertion mutation of AtLrgB produced plants with interveinal chlorotic and premature necrotic leaves."
Loss of the plastid envelope protein AtLrgB causes spontaneous chlorotic cell death in Arabidopsis thaliana.
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atlrgB Ds mutants show chlorotic cell death resembling lesion mimics; AtLrgB is expressed in leaves, stems and flowers but not roots.
"AtLrgB is expressed in leaves, stems and flowers, but not in roots"
Photorespiration.
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Before PLGG1 was identified, a single chloroplast carrier exchanging glycolate for glycerate had been characterized biochemically in pea and spinach.
"The transporter exchanged glycolate for glycerate ( Howitz and McCarty, 1991 )."
PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters.
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plgg1-1 is a high-CO2-requiring photorespiratory mutant that accumulates glycolate and glycerate; chloroplast O2-evolution, liposome uptake and 18O2 flux assays show PLGG1 transports glycolate and glycerate across the envelope.
"Our results indicate that PLGG1 is the chloroplastidic glycolate/glycerate transporter, which is required for the function of the photorespiratory cycle."
Physiological evidence for plasticity in glycolate/glycerate transport during photorespiration.
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plgg1-1 photorespires with wild-type efficiency under low light, implying alternative glycolate/glycerate transport routes.
"These alternative transport processes are adequate to maintain wild-type rates of net assimilation when net photorespiration is low as found under sub-saturating irradiance."
Bile Acid Sodium Symporter BASS6 Can Transport Glycolate and Is Involved in Photorespiratory Metabolism in Arabidopsis thaliana.
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PLGG1 rescues glycolate uptake in a yeast ady2 mutant; BASS6 and PLGG1 share glycolate export, while PLGG1 alone imports glycerate.
"Our data indicate that BASS6 and PLGG1 partner in glycolate export from the chloroplast, whereas PLGG1 alone accounts for the import of glycerate."
Chloroplast protein PLGG1 is involved in abscisic acid-regulated lateral root development and stomatal movement in Arabidopsis.
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plgg1 alleles show altered ABA responses in lateral root growth, germination, stomatal movement and drought tolerance.
"we found that PLGG1 is involved in other major ABA responses, including ABA-inhibited seed germination, ABA-mediated stomatal movement, and drought tolerance"