ach1 notes

• ach1 is Q9UUJ9, the single Schizosaccharomyces pombe entry in PTHR43609:SF1. The PTHR43609 entry table places it with the fungal Ach1 proteins from Saccharomyces cerevisiae, Candida albicans, Neurospora crassa, and other ascomycetes.
• The PAINT family has two relevant nodes for this review. PANTHER:PTN000484586 carries GO:0003986 acetyl-CoA hydrolase activity, GO:0008775 acetate CoA-transferase activity, and GO:0006083 acetate metabolic process. PANTHER:PTN000484602 carries the Ascomycota-scoped GO:0005739 mitochondrion placement.
• The key biochemical update is Fleck and Brock's re-characterization of budding-yeast Ach1p: "Ach1p showed highest specific activity for the CoASH transfer from succinyl-CoA to acetate and only a minor acetyl-CoA-hydrolase activity." PMID:19298859
• This means the acetate CoA-transferase and acetate metabolism rows should be core ACCEPT, while the older acetyl-CoA hydrolase rows are defensible but secondary (KEEP_AS_NON_CORE) rather than false.
• Mitochondrial localization is supported three ways: S. pombe HDA from the Matsuyama ORFeome/YFP localization survey PMID:16823372, S. cerevisiae fractionation and immunofluorescence in Buu et al. PMID:12606555, and the PTN000484602 Ascomycota IBA.
• The UniProt SubCell cytoplasm row is only a broad ancestor relative to mitochondrion and mitochondrial matrix; the generic GO:0008410 CoA-transferase activity row is likewise only a broad parent beside GO:0008775.
• The ISO to GO:0008260 succinyl-CoA:3-oxo-acid CoA-transferase activity should be removed. Its donor, UniProtKB:P55809, is a 3-oxo-acid CoA-transferase source, while Ach1's supported reaction is succinyl-CoA plus acetate to succinate plus acetyl-CoA.