Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
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UniProt keyword "Toxin" maps to GO:0090729, but this is an over-annotation for T3SS effectors
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UniProt keyword "Glycosyltransferase" appropriately maps to GO:0016757
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UniProt keyword "Transferase" appropriately maps to GO:0016740
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UniProt keyword "Metal-binding" appropriately maps to GO:0046872
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors
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Identified ensconsin/MAP7 as a binding partner for NleB1 using quantitative mass spectrometry
"we confirmed that NleB1 and EspL interacted with ensconsin in a region that corresponded to its microtubule binding domain"
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Wild-type EPEC infection disrupts intracellular trafficking; ΔnleB1 mutants do not
"Ensconsin is an essential cofactor of kinesin-1 that is required for intracellular trafficking, and we demonstrated that intracellular trafficking was severely disrupted during wild type EPEC infections but not during infections with ΔnleB1 or ΔespL mutants"
NleB/SseK effectors from Citrobacter rodentium, Escherichia coli, and Salmonella enterica display distinct differences in host substrate specificity
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EHEC NleB1 blocks TNF-mediated NF-kappaB pathway activation
"SseK1, SseK3, EHEC NleB1, EPEC NleB1, and Crodentium NleB blocked TNF-mediated NF-κB pathway activation"
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EHEC NleB1 glycosylates host GAPDH at Arg197 and Arg200
"EHEC NleB1 glycosylated two GAPDH arginine residues, Arg197 and Arg200"
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EHEC NleB1 glycosylates FADD death domain protein
"C. rodentium NleB, EHEC NleB1, EPEC NleB1, and SseK2 glycosylated the FADD (Fas-associated death domain protein)"
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Glycosylation of GAPDH prevents GAPDH-TRAF2 interaction and inhibits NF-kappaB signaling
"these two residues were essential for GAPDH-mediated activation of TNF receptor-associated factor 2 ubiquitination"
High-Throughput Screening for Bacterial Glycosyltransferase Inhibitors
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Characterized kinetic parameters of NleB1 (Km = 379 uM, kcat = 50 s-1)
"The kinetic parameters of NleB1 (150 nM at 30°C) were calculated as follows: Vmax: 2,975.3 ± 125 RLU/min/μg protein; Km: 379 ± 43 μM; Kcat (s-1): 50, Kcat/Km (s-1, M-1): 130,703.4"
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DXD motif essential for Mn2+ coordination and catalytic activity
"an inactive form of NleB1 (NleB1-AAA) in which the aspartic acid residues required for Mn2+ stabilization were mutated to alanines"
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NleB1 glycosylates TRADD at R235
"NleB1 glycosylates human TRADD on R235, thereby blocking death domain interactions and disrupting tumor necrosis factor signaling"
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Inhibitors were not toxic to mammalian cells and did not cause macrophage death
"We also failed to observe significant macrophage death in our studies"