Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast.
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Apl2 is the beta subunit of S. pombe AP-1; it binds Apm1 independently of Aps1 and Apl4, and all four subunits are needed for complex formation, endosomal association and endosomal exit of Syb1.
"In pull-down assay, Apm1 binds Apl2 even in the absence of Aps1 and Apl4"
Sip1, a conserved AP-1 accessory protein, is important for Golgi/endosome trafficking in fission yeast.
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GFP-Apl2 localizes to FM4-64-positive Golgi/endosomes in a Sip1-dependent manner, and Sip1 binds Apl2 in GST pull-downs.
"Results showed that the Sip1 protein bound to each of the subunits of the AP-1 complex"
Role of the Small GTPase Rho3 in Golgi/Endosome trafficking through functional interaction with adaptin in Fission Yeast.
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Rho3 overexpression suppresses Δapl2 phenotypes, and Rho3 binds GST-Apl2 in vitro.
"The results showed that the Rho3 protein bound to each of the subunits of the AP-1 complex"
Sip1, an AP-1 accessory protein in fission yeast, is required for localization of Rho3 GTPase.
Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast.
UniProt entry O43079 (apl2)
apl2 sequence-feature analysis (clathrin box, domain layout)
NCU09721 beta-adaptin placement re-review analysis (MAFFT/NJ tree including S. pombe apl2 and apl1)