PMID:18344366

Catalytic mechanism of cyclic di-GMP-specific phosphodiesterase: a study of the EAL domain-containing RocR from Pseudomonas aeruginosa.

Purified RocR hydrolyses c-di-GMP by a Mg2+-assisted general-base mechanism.

"catalyzes the hydrolysis of cyclic di-GMP by using a general base-catalyzed mechanism with the assistance of Mg(2+) ion"

PMID:19376848

The functional role of a conserved loop in EAL domain-based cyclic di-GMP-specific phosphodiesterase.

Loop 6 and the receiver-domain phosphorylation site modulate RocR PDE activity.

"the catalytic activity of RocR was affected by mutating the putative phosphorylation site (D56N) in the phosphoreceiver domain"

PMID:16477007

Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.

Comprehensive mutant and overexpression analysis of P. aeruginosa DGC/PDE genes; lysates of strains overexpressing RocR degraded all the added c-di-GMP (Table 1, read in the PMC full text).

"Analysis of the phenotypes of DGC and PDE mutants and overexpressing clones revealed that certain virulence-associated traits are controlled by multiple DGCs and PDEs through alterations in c-di-GMP levels."

PMID:15659157

A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes.

RocR antagonises RocA1, the DNA-binding activator of cup genes.

"RocA1 is a DNA binding response regulator that activates cup genes, and RocR is an antagonist of RocA1 activity"

PMID:15687209

A three-component regulatory system regulates biofilm maturation and type III secretion in Pseudomonas aeruginosa.

The SadARS (RocS1-RocA1-RocR) system is required for late biofilm maturation and regulates type III secretion genes.

"We propose that the sadARS three-component system is required for later events in biofilm formation on an abiotic surface."

PMID:22753070

Structural insights into the regulatory mechanism of the response regulator RocR from Pseudomonas aeruginosa in cyclic Di-GMP signaling.

RocR is a soluble tetramer in the crystal and in solution.

"a finding consistent with the observation that RocR forms tetramers in solution"

PMID:16980588

Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.

The degenerate EAL domain of E. coli CsrD does not hydrolyse c-di-GMP.

"These results suggested that the EAL domain of CsrD activity does not hydrolyze c-di-GMP."

file:PSEAE/rocR/rocR-notes.md

rocR review notes

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

GO_REF:0000033

Annotation inferences using phylogenetic trees