Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
UniProtKB A0BFB4 sequence and domain record
Falcon research on A0BFB4/GSPATT00028266001
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Target substrates are unresolved despite a supported catalytic architecture.
"The specific substrates of A0BFB4 are unknown."
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The provider does not establish a target pathway or localization experimentally.
"No direct localization data exist for protein A0BFB4."
A comparative in-silico analysis of autophagy proteins in ciliates.
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Ciliate genomes, including Paramecium tetraurelia, lack typical Atg1 proteins with the C-terminal domain needed for Atg1-complex assembly.
"ciliate genomes do not encode typical Atg1 since all the candidate sequences lack an Atg1-specific C-terminal domain which is essential for Atg1 complex formation"
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Ciliates also lack Atg1-complex partners, supporting lineage-level loss of the canonical Atg1 initiation machinery.
"Consistent with the absence of Atg1, ciliates also lack other members of the Atg1 complex."
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Ciliate Atg1-like kinase-domain proteins should not be treated as Atg1 without experiments, and whether any of them acts in ciliate autophagy is an open question.
"However, it is still an open question whether any of these kinase domain containing proteins have role(s) in autophagic processes in ciliates."
Role of class III phosphatidylinositol 3-kinase during programmed nuclear death of Tetrahymena thermophila.
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Tetrahymena programmed nuclear death is a ciliate autophagy-like process, supporting residual uncertainty about noncanonical ciliate autophagy regulation.
"Programmed nuclear death (PND) in the ciliate protozoan Tetrahymena thermophila is a novel type of autophagy that occurs during conjugation"
A0BFB4 verified PAINT descent and sequence-family discrepancy
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The actual tree places A0BFB4 below the autophagy-bearing ancestor; this is not merely a donor-list inference.
"The actual tree therefore confirms that A0BFB4 descends from the autophagy-bearing ancestral node."
OpenScientist focused report on A0BFB4 autophagy ancestry versus family placement
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The focused report found that PTHR44167 is a generic best-HMM hit rather than a competing family placement, leaving A0BFB4 in the Atg1/ULK clade.
"A0BFB4 genuinely belongs to the ATG1/ULK clade by ancestry, and this ancestry is **not** a misplacement."
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The focused report interpreted the autophagosome-assembly, regulation-of-autophagy, phagophore-assembly-site, and autophagosome IBAs as over-specific because the ciliate lineage and the A0BFB4 protein lack the canonical Atg1/ULK initiation-complex module.
"Because the machinery that nucleates a phagophore is absent in the ciliate lineage and in this protein specifically, GO:0000045, GO:0000407, GO:0005776, and GO:0010506 rest solely on phylogenetic IBA inference and cannot be executed by the canonical mechanism."
A0BFB4 kinase-domain placement and catalytic motifs (2026-10-21 re-review)
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A0BFB4 retains all canonical kinase catalytic motifs.
"All canonical kinase catalytic motifs are present in A0BFB4"
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A0BFB4 does not group robustly with Atg1/ULK kinases in a kinase-domain NJ tree.
"bootstrap support for A0BFB4 + ATG1/ULK is 1/200"