Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.
Sirtuin functions in health and disease.
Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5.
The first identification of lysine malonylation substrates and its regulatory enzyme.
Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase.
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SIRT5 demalonylation and desuccinylation are 29 to >1000-fold more efficient than deacetylation.
"With all three peptide sequences, the catalytic efficiencies for demalonylation and desuccinylation were much (29 to >1000 fold) higher than that for deacetylation"
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CPS1 acetylation at K44/K287 is unchanged in Sirt5 KO liver.
"For Lys44 and Lys287, the levels of acetylation and succinylation did not change significantly in Sirt5 KO mice"
SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.
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A significant fraction of endogenous SIRT5 is cytosolic in human cells.
"Subcellular fractionation revealed that a significant fraction of endogenous SIRT5 was present cytosolically both in human cells"
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SIRT5 deficiency does not change global lysine acetylation.
"SIRT5 deficiency does not significantly impact global Lys acetylation"
SIRT5 desuccinylates and activates SOD1 to eliminate ROS.
Lysine glutarylation is a protein posttranslational modification regulated by SIRT5.
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SIRT5 is the only HDAC with significant deglutarylase activity in vitro.
"Using this assay, we found SIRT5 is the only HDAC exhibiting significant lysine deglutarylase activity in vitro"
SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
SIRT5 deacetylates Cytochrome C
SIRT5 deglutarylates CPS1
UniProt record for human SIRT5