Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A conserved clathrin assembly motif essential for synaptic vesicle endocytosis.
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Bovine AP2 assembly and squid presynaptic intervention support conserved complex-level synaptic-vesicle retrieval, not autonomous human μ2 assembly chemistry.
"Microinjection of these peptides into squid giant
presynaptic terminals reversibly blocked synaptic transmission and inhibited
synaptic vesicle endocytosis by preventing coated pit formation at the plasma
membrane."
Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery.
Molecular architecture and functional model of the endocytic AP2 complex.
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μ2 is a structural and cargo-recognition subunit of the AP2 tetramer.
"We describe
the structure of the 200 kDa AP2 "core" (alpha trunk, beta2 trunk, mu2, and
sigma2)"
The mu2 subunit of the clathrin adaptor AP-2 binds to FDNPVY and YppØ sorting signals at distinct sites.
The AP-2 complex is excluded from the dynamic population of plasma membrane-associated clathrin.
Analysis of the AP-2 adaptor complex and cargo during clathrin-mediated endocytosis.
Towards a proteome-scale map of the human protein-protein interaction network.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
The dyslexia-associated protein KIAA0319 interacts with adaptor protein 2 and follows the classical clathrin-mediated endocytosis pathway.
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Human μ2 cargo-tail experiments link KIAA0319 Tyr995 recognition to receptor internalization.
"We
also identify tyrosine-995 of KIAA0319 as a critical amino acid required for the
interaction with AP-2 and subsequent internalization."
Quantitative proteomics identifies a Dab2/integrin module regulating cell migration.
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μ2 depletion in human HeLa cells increases surface integrins, supporting the existing internalization/localization role.
"AP2 or clathrin depletion significantly increases surface α1, α5, and β1."
Regulation of epidermal growth factor receptor trafficking by lysine deacetylase HDAC6.
Protein interactome reveals converging molecular pathways among autism disorders.
Identification and targeting of an interaction between a tyrosine motif within hepatitis C virus core protein and AP2M1 essential for viral assembly.
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Human AP2M1 recognizes the HCV core tyrosine sorting motif in direct and cell-based assays; both tested isoforms bind.
"Using microfluidics affinity analysis, protein-fragment
complementation assays, and co-immunoprecipitations in infected cells, we show
that this motif mediates core binding to AP2M1."
Oestrogen promotes KCNQ1 potassium channel endocytosis and postendocytic trafficking in colonic epithelium.
Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer's disease.
The identification of novel proteins that interact with the GLP-1 receptor and restrain its activity.
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The human GLP1R interactor screen and reported mammalian validation identify AP2M1 as a receptor partner; detailed primary Methods remain unavailable in this cache.
"A screen of a
human fetal brain cDNA prey library with an unliganded human GLP-1R as bait in
yeast revealed 38 novel interactor protein candidates. These interactions were
confirmed in mammalian Chinese hamster ovarian cells by coimmunoprecipitation."
Perturbation of the mutated EGFR interactome identifies vulnerabilities and resistance mechanisms.
TBC1D5 and the AP2 complex regulate ATG9 trafficking and initiation of autophagy.
CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
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μ2 depletion changes coated-pit/vesicle abundance and size; this does not assign CALM membrane-insertion chemistry to AP2M1.
"AP2 depletion (of μ2) resulted in an ∼12-fold decrease in the number of endocytic CCPs/CCVs"
Weak Molecular Interactions in Clathrin-Mediated Endocytosis.
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
A Recurrent Missense Variant in AP2M1 Impairs Clathrin-Mediated Endocytosis and Causes Developmental and Epileptic Encephalopathy.
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Arg170Trp impairs transferrin uptake in depletion/rescue models while coat localization remains preserved; reagent and host species are distinguished.
"Functional complementation of both the μ-subunit carrying the p.Arg170Trp
variant in human cells and astrocytes derived from AP-2μ conditional knockout
mice revealed a significant impairment of CME of transferrin."
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
Endocytic Adaptor Proteins in Health and Disease: Lessons from Model Organisms and Human Mutations.
Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D).
A reference map of the human binary protein interactome.
Cytoplasmic short linear motifs in ACE2 and integrin β(3) link SARS-CoV-2 host cell receptors to mediators of endocytosis and autophagy.
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ACE2 has a biochemically validated AP2 μ2 recognition site overlapping a phosphorylation-regulated binding region. Independently read original Tables/Methods resolve human μ2 and the ATG9A probe.
"a binding site for the
clathrin adaptor AP2 μ2 in ACE2 overlaps with a phospho-dependent binding site
for the SH2 domains of Src family tyrosine kinases."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities.
AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.
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Human CLDN2 motif mutants reduce AP2M1 association, connecting sorting-signal recognition with endocytic delivery.
"Site-directed mutagenesis revealed that AP2M1 binds to CLDN2 tyrosine motifs
(YXXФ) (67-70 and 148-151)."
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Physical and functional interactome atlas of human receptor tyrosine kinases.
Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs.
Large-scale phosphomimetic screening identifies phospho-modulated motif-based protein interactions.
Systematic identification of post-transcriptional regulatory modules.
Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2.
Sequence requirements for the recognition of tyrosine-based endocytic signals by clathrin AP-2 complexes.
Regulatory interactions in the recognition of endocytic sorting signals by AP-2 complexes.
Formation of CD4:Nef:AP-2 Complex:v-ATPase Complex
Internalization of the CD4:Nef:AP-2 Complex:v-ATPase Complex
Formation of Nef:Cd28:Clathrin-coated Pit Adapter Protein complex
Internalization of Nef:CD28:Clathrin-Coated Pit Adapter Protein Complex
Axonal transport of NGF:Trk complexes
Formation of CD8:Nef:AP-2 Complex:v-ATPase Complex
Internalization of the CD8:Nef:AP-2 Complex:v-ATPase Complex
Internalization of gap junction plaques
Lysosomal degradation of gap junction plaques
Dynamin is recruited to the gap junction plaque
Dab2 is recruited to the junctional plaques
Uncoating of clathrin-coated vesicles and fusion with endosomes
Recruitment of clathrin coated vesicle by Ii
Internalization of MHC II:Ii clathrin coated vesicle
L1 binds to AP-2 Clathrin complex
Transport of L1 into endosomes
Clathrin internalises EPH:EFN complexes
Trafficking of GluR2-containing AMPA receptors to extrasynaptic sites
Reinsertion of L1 into the plasma membrane
Phosphorylation of L1 by ERK
p-DVL2 recruits AP-2 and beta-arrestin 2 to promote clathrin-mediated endocytosis
WNT5A:FZD4 is endocytosed
Formation of clathrin coated vesicle
PCSK9:LDLR:Clathrin-coated vesicle transport from plasma membrane to endolysosome
PCSK9:LDLR bind to Clathrin
Degradation of PCSK9:LDLR:Clathrin-coated vesicle
VLDLR:PCSK9:Clathrin-coated vesicle translocates from the plasma membrane to lysosomal membrane
VLDLR:PCSK9 binds Clathrin-coated vesicles
Recruitment of AP-2 complex and clathrin
AAK1 phosphorylates AP-2 mu subunit at T156
FCHo proteins bind nascent clathrin-coated pit
AP-2 directly binds some endocytic cargo
Epsin family proteins bind ubiquitinated cargo
ARBB recruits GPCRs into clathrin-coated pits
F- and N- BAR domain proteins bind the clathrin-coated pit
CLASP proteins and cargo are recruited to the nascent clathrin-coated pit
Clathrin recruits PIK3C2A
Clathrin-associated PIK3C2A phosphorylates PI(4)P to PI(3,4)P2
SNX9 recruits components of the actin polymerizing machinery
BAR domain proteins recruit dynamin
SYNJ hydrolyze PI(4,5)P2 to PI(4)P
Endophilins recruit synaptojanins to the clathrin-coated pit
HSPA8-mediated ATP hydrolysis promotes vesicle uncoating
Clathrin recruits auxilins to the clathrin-coated vesicle
Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle
Dynamin-mediated GTP hydrolysis promotes vesicle scission
Dissociation of clathrin-associated proteins
Dissociation of AAK1 and dephosphorylation of AP-2 mu2
RAB5 and GAPVD1 bind AP-2
Initial binding of AP-2 and clathrin to PI(4,5)P2
Functional analysis of AP-2 alpha and mu2 subunits.
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μ2 cargo-site and phosphorylation-site mutations impair transferrin uptake in HeLa replacement assays.
"Mutating the PtdIns(4,5)P2 binding site of
alpha, the phosphorylation site of mu2, or the YXXPhi binding site of mu2
impairs AP-2 function, as assayed by transferrin uptake."
A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex.
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The AP2 complex couples membrane and cargo binding to the clathrin scaffold through a regulated structural rearrangement.
"The AP2 adaptor complex (alpha, beta2, sigma2, and mu2 subunits) crosslinks the
endocytic clathrin scaffold to PtdIns4,5P(2)-containing membranes and
transmembrane protein cargo."
Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.
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μ2 Thr156 phosphorylation creates a NECAP-recruitment platform during coat maturation.
"μ2Thr156 phosphorylation favors a new, cargo-bound conformation of AP2
and simultaneously creates a binding platform for the endocytic NECAP proteins"
Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.
SLC26A4-AP-2 mu2 interaction regulates SLC26A4 plasma membrane abundance in the endolymphatic sac.
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Mouse cargo-tail/μ2 assays support recognition of SLC26A4; human host cells and structural models are distinct from construct species.
"the elements involved in SLC26A4-μ2 interaction were identified and validated
experimentally"