Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Identification and characterization of FTSJ2, a novel human nucleolar protein homologous to bacterial ribosomal RNA methyltransferase.
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FTSJ2 was cloned as a homologue of E. coli FtsJ/RrmJ, a 23S rRNA uridine-2'-O-methyltransferase; immunofluorescence suggested nucleolar localisation.
"Immunofluorescence staining demonstrated that FTSJ2 protein localizes to the nucleolus."
Mitochondrial ribosomal RNA (rRNA) methyltransferase family members are positioned to modify nascent rRNA in foci near the mitochondrial DNA nucleoid.
MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome.
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Endogenous MRM2 cofractionates with the matrix protein mtSSB1 and is protease-protected; knockdown causes aberrant mtLSU assembly and respiratory incompetence.
"Ineffective translation in MRM2- and MRM3-depleted cells results from aberrant assembly of the large subunit of the mitochondrial ribosome (mt-LSU)."
Assignment of 2'-O-methyltransferases to modification sites on the mammalian mitochondrial large subunit 16 S ribosomal RNA (rRNA).
Defective mitochondrial rRNA methyltransferase MRM2 causes MELAS-like clinical syndrome.
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Homozygous MRM2 p.Gly189Arg causes a MELAS-like disease; the equivalent yeast mrm2 allele fails to rescue respiration.
"Complementation with the mrm2 allele carrying the equivalent yeast mutation failed to rescue the respiratory phenotype, which was instead completely rescued by expressing the wild-type allele."
Human GTPBP5 (MTG2) fuels mitoribosome large subunit maturation by facilitating 16S rRNA methylation.
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GTPBP5 interacts with MRM2 and facilitates Um1369 installation; MRM2 knockdown gives fully unmodified Um1369.
"The GTPBP5 interaction with MRM2 was also detected using the proximity-dependent biotinylation (BioID) assay."
Structural basis for late maturation steps of the human mitoribosomal large subunit.
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MRM2 is trapped on GTPBP5-deficient mtLSU intermediates; MTERF4 enables MRM2 binding.
"MTERF4 binding of pre-H68-71 partly orders the disordered rRNA in the mt-LSU assemblies’ subunit interface side and thereby enables MRM2 to bind"
Stepwise maturation of the peptidyl transferase region of human mitoribosomes.
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NSUN4-MTERF4 exposes 16S rRNA regions for modification by MRM2 in human mtLSU assembly intermediates.
"exposes the functionally important regions of rRNA for modification by the MRM2 methyltransferase"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit.
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MRM2 is required for Um1369 (U3039) and acts as a catalysis-independent late-stage mtLSU assembly factor; catalytic mutants K59A/D154A rescue translation.
"However, mitoribosome biogenesis does not depend on the methyltransferase activity of MRM2."
Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome.
MRM2 (FTSJ2) methylates uridine-1369 of 16S rRNA yielding 2'-O-methyluridine
UniProt entry Q9UI43 (MRM2_HUMAN)