Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Analysis of DRAM-related proteins reveals evolutionarily conserved and divergent roles in the control of autophagy.
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DRAM2 is a lysosomal protein
"DRAM2 is also a lysosomal protein"
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DRAM2 shows 45% identity and 67% conservation compared to DRAM1
"The closest of these, which we have termed DRAM2, displays 45% identity and 67% conservation when compared to DRAM"
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DRAM2 is not induced by p53 or p73 (unlike DRAM1)
"DRAM2 is different from DRAM as it not induced by p53 or p73"
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DRAM2 overexpression alone does not modulate autophagy (unlike DRAM1)
"DRAM2 is also a lysosomal protein, but again unlike DRAM its overexpression does not modulate autophagy"
Reduced expression of DRAM2/TMEM77 in tumor cells interferes with cell death.
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DRAM2 is mainly localized in the lysosome
"DRAM2 is mainly localized in the lysosome, and co-localizes with DRAM"
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DRAM2 co-localizes with DRAM1
"DRAM2 is mainly localized in the lysosome, and co-localizes with DRAM"
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DRAM2 expression is down-regulated in ovarian tumors
"the expression of DRAM2 is down-regulated in ovarian tumors"
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DRAM2 alone does not induce cell death
"While expression of DRAM or DRAM2 individually did not induce cell death"
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Co-expression of DRAM2 with DRAM1 induces cell death
"co-expression of DRAM2 with DRAM significantly induced cell death"
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DRAM2 knockdown attenuates cell death
"silencing of endogenous DRAM2 attenuated cell death"
Biallelic mutations in the autophagy regulator DRAM2 cause retinal dystrophy with early macular involvement.
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Biallelic DRAM2 mutations cause cone-rod dystrophy 21 (CORD21)
"Affected individuals were found to harbor disease-causing variants in DRAM2"
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Disease characterized by adult-onset retinal dystrophy with early macular involvement
"five families affected by an adult-onset retinal dystrophy with early macular involvement"
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Visual loss typically occurs in third or fourth decade of life
"associated central visual loss in the third or fourth decade of life"
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DRAM2 localizes to photoreceptor inner segments
"Immunohistochemical analysis showed DRAM2 localization to photoreceptor inner segments"
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DRAM2 localizes to apical surface of retinal pigment epithelial cells
"Immunohistochemical analysis showed DRAM2 localization to photoreceptor inner segments and to the apical surface of retinal pigment epithelial cells"
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DRAM2 may be involved in photoreceptor renewal and recycling
"where it might be involved in the process of photoreceptor renewal and recycling to preserve visual function"
Deep research review of DRAM2 function
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DRAM2 deficiency causes impaired autophagic flux
"DRAM2 deficiency led to impaired autophagic flux (blunted LC3-II changes with bafilomycin/rapamycin)"
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DRAM2 loss causes lysosomal enzyme deficiencies
"reduced activities of key lysosomal enzymes (cathepsin D, GBA, a-mannosidase; severe PPT1/NPC2 deficiency with extracellular mis-secretion)"
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DRAM2 co-localizes with AP-1 and AP-3 adaptor complexes
"Proteomics indicated downregulation of clathrin adaptors AP-1/AP-3, with DRAM2 co-staining with these adaptors"