Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
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Each of the two seven-gene phz operons of PAO1 is sufficient for phenazine-1-carboxylic acid production in a phenazine-non-producing pseudomonad; this operon-level experiment is the basis of the PseudoCAP IDA rows on all the phz genes, phzC1 among them.
"Functional studies of phenazine-nonproducing strains of fluorescent pseudomonads indicated that each of the biosynthetic operons from P. aeruginosa is sufficient for production of a single compound, phenazine-1-carboxylic acid (PCA)."
Structural and functional characterisation of the entry point to pyocyanin biosynthesis in Pseudomonas aeruginosa defines a new 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase subclass.
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The phz-cluster DAH7PS of PAO1 (PA1901, identical to PA4212) was crystallised and kinetically characterised; it is a distinct subclass of type II DAH7PS that lacks the helices carrying the allosteric site, and it is not inhibited by aromatic amino acids, phenazine or PCA.
"Consistent with this is the observation made during functional characterisation that PaeDAH7PSPA1901 is insensitive to allosteric regulation by aromatic amino acids, confirming that PaeDAH7PSPA1901 functions primarily within secondary metabolism."
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The same work distinguishes this enzyme from the organism's housekeeping DAH7PS PA2843, which does carry the allosteric helices.
"A primary structure alignment among type II DAH7PSs (Supplementary Figure S1) indicates that PaeDAH7PSPA1901 appears to lack much of the sequence that corresponds to the"
Uncovering the Role of PhzC as DAHP Synthase in Shikimate Pathway of Pseudomonas chlororaphis HT66.
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In P. chlororaphis HT66, deleting phzC sharply reduces phenazine-1-carboxamide production and complementation restores it, while deleting the housekeeping aroF, aroG and aroH genes has little effect.
"After the complementation of gene phzC in the corresponding DAHP synthase-deficient strain, the production of PCN was significantly restored."
UniProt entry Q7DC82 (PA1901 / PA4212, phzC1/phzC2)
phzC1 bioinformatics - PAINT node PTN000481936 composition and propagation