TIA1 GO Annotation Breakdown

Summary by GO Aspect

Molecular Function (MF) - 12 annotations

GO ID Term Action Evidence Key Notes
GO:0140517 protein-RNA adaptor activity ACCEPT IBA Core adaptor function
GO:0003676 nucleic acid binding MODIFY→GO:0003723 IEA Too general
GO:0003723 RNA binding ACCEPT (4×) IEA, IDA, HDA(2×) Core function, multiple confirmations
GO:0003730 mRNA 3'-UTR binding ACCEPT (2×) IEA, ISS 3'UTR binding for translation regulation
GO:0035925 mRNA 3'-UTR AU-rich region binding ACCEPT IEA Most specific 3'UTR binding term
GO:0008143 poly(A) binding ACCEPT TAS Historical characterization
GO:0005515 protein binding MODIFY (5×) IPI Too general, replace with GO:0140517

MF Summary: Core functions are RNA binding (U-rich/AU-rich specificity) and protein-RNA adaptor activity. Generic "protein binding" should be replaced with more informative terms.

Biological Process (BP) - 11 annotations

GO ID Term Action Evidence Key Notes
GO:0000381 regulation of alternative mRNA splicing, via spliceosome ACCEPT (4×) IBA, IEA, IDA(2×) Core function, best characterized
GO:0006397 mRNA processing ACCEPT IEA Broad but correct
GO:0008380 RNA splicing ACCEPT IEA Broad but correct
GO:0048024 regulation of mRNA splicing, via spliceosome ACCEPT (2×) IDA Intermediate specificity
GO:0006915 apoptotic process ACCEPT (2×) IEA, TAS Context-dependent function
GO:0017148 negative regulation of translation ACCEPT (2×) IEA, ISS Core cytoplasmic function
GO:0034063 stress granule assembly ACCEPT IDA Core stress response function
GO:1903608 protein localization to cytoplasmic stress granule ACCEPT IMP Active recruitment role

BP Summary: Three major biological processes - (1) alternative splicing regulation, (2) stress granule assembly and stress response, (3) translational repression. Apoptosis is context-dependent.

Cellular Component (CC) - 20 annotations

GO ID Term Action Evidence Key Notes
GO:0005634 nucleus ACCEPT (3×) IEA, IDA(2×) Primary location under normal conditions
GO:0005654 nucleoplasm ACCEPT (2×) IDA, TAS More specific than nucleus
GO:0005737 cytoplasm ACCEPT (3×) IEA, IDA(2×) Stress-induced translocation
GO:0005829 cytosol ACCEPT IDA Specific cytoplasmic compartment
GO:0010494 cytoplasmic stress granule ACCEPT (5×) IEA, IDA(2×), ISS Core stress response location
GO:0097165 nuclear stress granule ACCEPT (2×) IEA, IDA Less common but documented

CC Summary: Dynamic dual localization - nuclear (splicing) under normal conditions, cytoplasmic (stress granules, translation) under stress. Both cytoplasmic and nuclear stress granules documented.

Evidence Code Distribution

Evidence Code Count Interpretation
IEA 12 Automated computational inference
IDA 13 Direct experimental assay
IBA 2 Phylogenetic inference
ISS 3 Sequence similarity inference
IPI 5 Protein interaction
IMP 1 Mutant phenotype
TAS 3 Traceable author statement
HDA 2 High-throughput direct assay

Total: 41 evidence instances for 43 annotation lines (some have multiple instances of same term)

Curation Actions by Evidence Type

IEA (Electronic Annotation)

IDA (Direct Assay)

IPI (Protein Interaction)

Other Evidence (IBA, ISS, IMP, TAS, HDA)

Key Findings

  1. Highly confident annotations: IDA and IBA annotations are uniformly high quality
  2. Main curation issue: Generic "protein binding" term (5 instances) needs replacement
  3. No incorrect annotations: All terms represent valid aspects of TIA1 biology
  4. Well-balanced coverage: Good representation across all three GO aspects
  5. Evidence diversity: Mix of computational, experimental, and curated evidence
  6. Duplicate terms acceptable: Multiple instances of same GO term with different evidence codes provides robust support

Annotation Specificity Analysis

Optimal Specificity

Acceptable Broad Terms

Terms Requiring Replacement