Evidence that WapB is a 1,2-glucosyltransferase of Pseudomonas aeruginosa involved in Lipopolysaccharide outer core biosynthesis.
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The wapB mutant core oligosaccharide lacks the terminal beta-1,2-linked D-glucose, and complementation restores the wild-type structure.
"revealed that the core OS of the wapB mutant lacked the terminal β-1,2-linked-d-glucose residue."
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Expressing wapB in serotypes lacking Glc(IV) adds a terminal glucose, and the authors conclude WapB is a 1,2-glucosyltransferase.
"Our results strongly suggested that wapB encodes a 1,2-glucosyltransferase."