file:SCHPO/nce101/nce101-deep-research-falcon.md

Falcon deep research report for S. pombe nce101

The report distinguishes the 53-58-aa NCE101 family from the unrelated Nce102/MARVEL family and finds no direct functional characterization of S. pombe nce101.

file:SCHPO/nce101/nce101-hypotheses/nonclassical-protein-export-function/openscientist.md

OpenScientist hypothesis report on direct non-classical export by nce101

The report confirms that the S. pombe secretion annotations are transferred rather than direct. Its executive REFUTED label overstates what the report's own caveat concedes: lack of direct evidence does not demonstrate that nce101 is uninvolved in secretion. Comparisons to the unrelated NCE102 and NCE103 proteins are nomenclature context only.

file:interpro/panther/PTHR28011/PTHR28011-review.md

PANTHER family review PTHR28011: NCE101 protein-secretion propagation assessment

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000015

Use of the ND evidence code for Gene Ontology (GO) terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

PMID:11859360

The genome sequence of Schizosaccharomyces pombe.

PMID:18488015

Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution.

PMID:8655575

A new pathway for protein export in Saccharomyces cerevisiae.

Defines the non-classical (signal-sequence-independent) protein export pathway in budding yeast and, through a genetic screen, identified the founding NCE genes including NCE1/NCE101, the ortholog of S. pombe nce101. Establishes the family-level basis for the protein secretion / protein transport annotations, but the paper's molecular characterization is performed on NCE2, not on the small NCE1 gene product, whose role (machinery versus exported substrate) was left unresolved.

"A screen for components of the export machinery has identified genes that are involved in nonclassical export. These findings demonstrate a new pathway for protein export that is distinct from the classical secretory pathway in yeast."