GO_REF:0000104

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

Transfers the broad nucleotide binding term, which is redundant with the more specific ATP binding annotation.

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links

Correctly infers MAPK cascade involvement from the MAP kinase activity annotation via inter-ontology logical links.

GO_REF:0000116

Automatic Gene Ontology annotation based on Rhea mapping

Maps the ARBA-attached Rhea serine phosphorylation reaction to protein serine kinase activity.

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

ARBA assigns the broad signal transduction term, which is entailed by the more specific MAPK cascade annotation.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Recovers MAP kinase activity and ATP binding from InterPro signatures and EC mapping.

file:CUCME/A0A1S3BTE3/A0A1S3BTE3-uniprot.txt

UniProt entry A0A1S3BTE3

Unreviewed TrEMBL entry (PE 3), 492 aa; protein name, EC number, catalytic activities and family note are all ARBA-derived.
Matches MAPK-specific signatures (PROSITE PS01351, InterPro IPR003527, CDD STKc_TDY_MAPK) and the protein-kinase ATP-binding signature with binding site K54.

file:CUCME/A0A1S3BTE3/A0A1S3BTE3-bioinformatics/RESULTS.md

A0A1S3BTE3 phylogenetic placement and catalytic-motif check

Catalytic residues and the TDY activation-loop motif are intact; the protein is a group D MAPK.
In an NJ tree with all 20 Arabidopsis MPKs, it falls in the MPK18/MPK19/MPK20 clade, sister to MPK18+MPK19. The PANTHER subfamily of the target could not be determined.

PMID:32407323

Genome-wide identification of mitogen-activated protein kinase (MAPK) cascade and expression profiling of CmMAPKs in melon (Cucumis melo L.).

Identifies 14 melon MAPKs. CmMAPK19 (MELO3C016139, 492 aa, 56.6 kDa) is placed in group D, and its 492-aa length and MW match A0A1S3BTE3.

"the remaining 7 members were assigned to group D (CmMAPK9-1, CmMAPK9-2, CmMAPK9-4, CmMAPK16, CmMAPK19, CmMAPK20-1 and CmMAPK20-2)"
CmMAPK19 transcripts decreased under drought and after Podosphaera xanthii inoculation (qRT-PCR only).

"Under drought stress, except that the transcription levels of CmMAPK19, CmMAPK20-1 and CmMAPK20-2 decreased"

PMID:25976104

Genome-wide identification of MAPK, MAPKK, and MAPKKK gene families and transcriptional profiling analysis during development and stress response in cucumber.

In cucumber, group D MAPKs carry the TDY motif and lack the CD docking domain found in groups A-C.

"the members of group D contain a TDY (Thr-Asp-Tyr) motif and form a more distant clade"

PMID:19392697

Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells.

Arabidopsis MPK18 (group D, same clade as the target) interacts with the MAPK phosphatase PHS1 in the cytoplasm and is required for normal microtubule-related functions.

"Mutant mpk18 seedlings show defects in microtubule-related functions, and have moderately stabilized microtubules."

PMID:28848569

The Arabidopsis Mitogen-Activated Protein Kinase Kinase Kinase 20 (MKKK20) Acts Upstream of MKK3 and MPK18 in Two Separate Signaling Pathways Involved in Root Microtubule Functions.

MKKK20 phosphorylates MPK18 directly, a non-canonical module that bypasses a MAP2K.

"In vitro phosphorylation assays showed that MKKK20 phosphorylates both MKK3 and MPK18."