Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway.
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Recombinant Msed_0656 is an active acetoacetyl-CoA beta-ketothiolase with a Km of 0.18 mM for acetoacetyl-CoA.
"Msed_0656 on acetoacetyl-CoA, 0.18 mM"
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It is the fastest enzyme of the acetyl-CoA branch.
"more than 100-fold lower than that of the β-ketothiolase (Msed_0656) (140 μmol −1 min −1 mg −1 )"
Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota.
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Msed_0656 was assigned as the cycle beta-ketothiolase by similarity to the bacterial enzyme. The activity was demonstrated for the Thermoproteus ortholog Tneu_0249, not for Msed_0656 itself.
"that was highly similar to the eubacterial enzyme"
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Msed_0656 is upregulated in autotrophic growth.
"In M. sedula , Msed_0656 exhibited upregulation under autotrophic conditions"
Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO(2) Fixation in Metallosphaera sedula.
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Other thiolase paralogs, notably Msed_0386, are also induced in autotrophy and may contribute to acetoacetyl-CoA cleavage.
"as at least one of the homologous genes (msed_0386) is up-regulated under mixo- and autotrophic growth conditions"