Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Immunopurification and mass spectrometry of human Complex I identified NDUFB7 among the subunits of the holoenzyme, confirming physical membership.
"subunit composition of the human NADH dehydrogenase"
Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
NDUFB7 and NDUFA8 are located at the intermembrane surface of complex I.
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NDUFB7 and NDUFA8 are Cx9C-containing subunits localized at the intermembrane-space surface of Complex I; proposed to stabilize the membrane domain via intramolecular disulfide bridges.
"NDUFB7 and NDUFA8 are located at the intermembrane surface of complex I"
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Systematic knockout/quantitative-proteomics analysis of human Complex I accessory subunits (including NDUFB7) shows accessory subunits are integral for assembly and function; loss of a subunit destabilizes others in the same module.
"Accessory subunits are integral for assembly and function of human mitochondrial"
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM of the human megacomplex I2III2IV2 resolves the precise assignment of individual Complex I subunits, placing NDUFB7 within the holoenzyme.
"precise assignment of individual subunits of human CI"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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Review of OXPHOS complex assembly; Complex I comprises core catalytic subunits plus supernumerary subunits important for assembly, regulation and stability.
"assembly of the five oxidative phosphorylation system (OXPHOS) complexes"
A reference map of the human binary protein interactome.
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Proteome-wide binary (Y2H) interactome map (HuRI); the source of NDUFB7 IPI "protein binding" annotations to numerous non-Complex-I partners.
"reference interactome map of human binary protein interactions"
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Severe congenital lactic acidosis and hypertrophic cardiomyopathy caused by an intronic variant in NDUFB7.
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A biallelic intronic NDUFB7 variant caused reduced NDUFB7 protein and reduced Complex I activity; complementation with wild-type NDUFB7 normalized Complex I function, demonstrating NDUFB7 is required for holoenzyme activity (MC1DN39).
"resulted in a significant reduction of the NDUFB7 protein and reduced complex I activity"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Characterization of nuclear-encoded Complex I subunits in the hydrophobic-protein fraction; Complex I transports electrons from NADH to ubiquinone with proton translocation.
"transport of electrons from NADH to ubiquinone"
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex