Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by ARBA machine learning models
A Ycf2-FtsHi Heteromeric AAA-ATPase Complex Is Required for Chloroplast Protein Import.
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Arabidopsis biochemistry identifies the Ycf2-FtsHi motor containing FtsH12 and its association with translocating preproteins.
"a 2-MD heteromeric AAA-ATPase complex associates with the TIC complex and functions as the import motor, directly interacting with various translocating preproteins"
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H769Y complementation preserves viability and import, separating the essential motor-associated role from zinc-dependent proteolysis.
"even the FtsH12 zinc binding site is dispensable for its essential function"
Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.
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Fractionation and protease-protection experiments support inner-envelope localization with the ATPase/protease region facing the stroma.
"this unambiguously confirms the localization of FtsH12 in the inner chloroplast envelope"
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Proteome and N-terminome analyses do not establish FtsH12 as the general maturase for imported proteins.
"N-terminome analyses further demonstrated normal proteolytic maturation of
plastid-imported proteins irrespective of FTSH12 abundance."
Conservation of the FTSH12 zinc-site histidine
Frozen exact-input ProtNLM output and UniProt sequence for A0A1P8ARD2
Structural insights into the chloroplast protein import in land plants.
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The 2024 Arabidopsis structural work corroborates the heteromeric ATPase import-motor architecture; it does not establish a physiological FtsH12 protease substrate.
"The Ycf2-FtsHi structure reveals a
heterohexameric AAA+ ATPase motor module with characteristic features."