RfC3 curation notes
- RfC3 is an AAA+ activator-1 small subunit of replication factor C; ortholog of human RFC5 (UniProt name 'Activator 1 subunit 5').
- Little gene-specific experimental work exists; evidence is from the RFC/RLC literature and fly complex purification.
- RFC function (general): PMID:8999859; PMID:8999859.
- Elg1 complex membership in S2 cells: PMID:27198229. Elg1 complex unloads PCNA: PMID:27198229.
- PMID:24204884 (Elongin/Corto) cited by FlyBase NAS/IPI rows does not mention RFC (full text searched); flagged MISCITED; claims judged on RFC biology.
- Protein binding IPI rows are with other RFC small subunits (GOA WITH/FROM); removed as uninformative.
- Same decision table as RfC4/RfC3/RfC38/CG8142 (module consistency): contributes_to GO:0003689 and GO:0061860 (core), complexes GO:0005663 and GO:0031391.
- Round-2 rule applied: generic parents (DNA replication, ATP-dependent activity acting on DNA, protein-containing complex) changed from KEEP_AS_NON_CORE to MODIFY toward the specific term the gene already carries; subunit-level binding/ATPase terms stay KEEP_AS_NON_CORE.
- Falcon deep research (late): wing-disc RNAi of Rfc3 (Kohzaki 2018) gives wing phenotypes in all 158 flies; Rfc3 grouped with replication-elongation factors; complex-level functions otherwise inferred cross-species.
- Review-bot round: added NEW contributes_to GO:0061860 DNA clamp unloader activity (IDA, PMID:27198229) so the core-function claim has an annotation row; identical across the four small subunits.