Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniPathway vocabulary mapping
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Characterization of Pseudomonas aeruginosa l,d-Transpeptidases and Evaluation of Their Role in Peptidoglycan Adaptation to Biofilm Growth.
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Pseudomonas L,D-transpeptidase paralogs have experimentally distinct crosslinking, OprI-anchoring, and tether-hydrolysis functions.
"We found that the three P. aeruginosa LDTs catalyze peptidoglycan cross-linking (LdtPae1), the anchoring of lipoprotein OprI to the peptidoglycan (LdtPae2), and the hydrolysis of the resulting peptidoglycan-OprI amide bond (LdtPae3)."
Outer Membrane-Peptidoglycan Anchoring in Pseudomonas aeruginosa.
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PA2854 directly anchors OprI to peptidoglycan in vivo and with purified components in vitro.
"We document that the gene product of PA2854 is the catalyst that performs this transformation between the cell wall and the outer-membrane lipoprotein OprI in live Pseudomonas aeruginosa."
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The PA2854 reaction was reconstituted with purified PA2854, OprI, and synthetic peptidoglycan.
"Furthermore, we reconstitute this reaction in vitro with the use of purified recombinant PA2854, OprI, and synthetic samples of the cell-wall peptidoglycan, in each case documenting the attachment of the side-chain ε-amino group of Lys83 of OprI to the peptide stem of the peptidoglycan."
OpenScientist deep research report on PSEPK PP_2320 (Q88KH0)
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The report identifies PP_2320 as the periplasmic ortholog of the OprI-anchoring Pseudomonas L,D-transpeptidase.
"PP_2320 is the *P. putida* ortholog (76% identity) of PA2854, the OprI-anchoring L,D-transpeptidase"