Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii
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Deletion mutants fail to accumulate nitrogenase structural gene products
"Mutant strains that have a deletion or a deletion plus an insertion within nifA are incapable of diazotrophic growth and they fail to accumulate nitrogenase structural gene products."
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NifA contains conserved regions with consensus ATP binding site
"Interspecies comparisons of nifA-encoded products reveal that they all have a consensus ATP binding site and a consensus DNA binding site in highly conserved regions of the respective polypeptides."
Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii
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nifA is separated from nifB by a 285-bp regulatory region
"A 285-base-pair sequence containing a potential nif promoter and nif regulatory sites separates this nifA gene from the first complete ORF which encodes a protein homologous to nifB gene products"
UniProt entry for NifA from Azotobacter vinelandii
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Required for activation of most nif operons involved in nitrogen fixation
"FUNCTION: Required for activation of most nif operons, which are directly involved in nitrogen fixation"
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Interacts with sigma-54
"SUBUNIT: Interacts with sigma-54"
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Contains GAF domain (37-178), sigma-54 interaction domain (211-439), and HTH DNA-binding domain (494-513)
"DOMAIN 37..178 /note="GAF" ... DOMAIN 211..439 /note="Sigma-54 factor interaction" ... DNA_BIND 494..513 /note="H-T-H motif""
The CbrB Regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida.
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In sigma-54 enhancer-binding proteins, AAA+ hexamerisation is required for ATPase activity and for remodelling the closed promoter complex into an open complex (family-level background, not NifA-specific)
"Hexamerisation is required both for ATPase activity and for interaction with the holoenzyme bound at the promoter and remodelling the closed complex into a transcriptionally competent open complex"
Gene Ontology annotation through association of InterPro records with GO terms.
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods.