RLF2 curation notes

2026 IBA re-review

Re-checked all six current RLF2/CAC1 IBA annotations against GOA and the cached
PTHR15272 PAINT table. Every row traces to the same eukaryotic CAF-1 subunit A
ancestral node, PANTHER:PTN000392234:

• GO:0000510 H3-H4 histone complex chaperone activity
• GO:0000785 chromatin
• GO:0005634 nucleus
• GO:0006334 nucleosome assembly
• GO:0006335 DNA replication-dependent chromatin assembly
• GO:0033186 CAF-1 complex

The current actions are sound. Cac1/Rlf2 is the large scaffold subunit of yeast
CAF-1, is part of CAF-1, carries histone- and DNA-binding surfaces, and acts in the
nucleus on chromatin to deposit H3-H4 during replication-coupled assembly. The YAML
already had GOA supporting_entities; this pass adds propagation_review blocks
that point at the PTN ancestral node as the propagation source and mark all six
rows as NO_FAILURE_CORE.

The newer-paper search found a 2026 centromeric-H3-variant review mentioning yeast
CAF-1/Cac1 PMID:41993598 and the 2024 fission-yeast CAF-1 structural work already discussed in
the Falcon report PMID:38376141, but no newer direct budding-yeast Cac1 paper that changes these IBA
calls.

PR follow-up

Reviewer follow-up added PubMed-backed provenance for the newer-paper sweep, using the
2026 Frontiers Cse4 review and the 2024 eLife fission-yeast CAF-1 structural paper.
The H3-H4 chaperone IBA now records the single fission-yeast Pcf1 donor explicitly,
and the CAF-1-complex IBA records the RLF2 self-seed as target evidence rather than
circular support.